Variation among Colletotrichum gloeosporioides isolates from infected coffee berries at different locations in Vietnam |
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Authors: | T H P Nguyen T Säll T Bryngelsson E Liljeroth |
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Institution: | Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE 230 53 Alnarp;;Department of Cell and Organism Biology (Genetics), Lund University, SE 223 62 Lund, Sweden;;Department of Plant Molecular Pathology and Biological Control, Institute of Agricultural Genetics and Biotechnology (AGBI), Pham Van Dong Street, Tuliem, Hanoi, Vietnam;;and Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, SE 230 53 Alnarp, Sweden |
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Abstract: | Isolates of Colletotrichum gloeosporioides associated with anthracnose disease on coffee berries in Vietnam were characterized by morphological and molecular methods. Random amplified polymorphic DNA (RAPD) and microsatellite-primered PCR (MP-PCR) analyses were employed to investigate the genetic variation among 38 and 51 isolates of C. gloeosporioides , respectively. According to both methods, the isolates mainly grouped in accordance with geographical origins. Higher genetic variation ( H = 0·312 and 0·335) in the northern population of C. gloeosporioides than in the southern population ( H = 0·261 and 0·186), according to the RAPD and MP-PCR markers, respectively, was indicative of a difference between the northern and southern populations. Moderate gene differentiation ( G st = 0·1) between populations from the north and the south was found. However, there was no differentiation between locations within the northern or southern populations, indicating significant gene flow. A four-gamete test indicated a high level of recombination, particularly in the south. The geographic differences may be explained by different histories of coffee cultivation in different parts of Vietnam. The symptoms caused by the Vietnamese isolates on both hypocotyls and green berries were less severe than symptoms caused by the reference CBD (coffee berry disease; Colletotrichum kahawae ) isolates originating from Africa. |
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Keywords: | anthracnose disease Coffea spp genetic variation MP-PCR pathogenicity RAPD |
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