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| 石蒜组培繁殖技术的研究 | |
| 引用本文： | 朱锦,诸葛强,余水生,陈宁军.石蒜组培繁殖技术的研究[J].浙江林业科技,2002,22(4):45-48. |
| 作者姓名： | 朱锦 诸葛强 余水生 陈宁军 |
| 作者单位： | 1. 南京林业大学,江苏,南京,210037 2. 浙江省遂昌县林业局,浙江,遂昌,323300 3. 浙江省兰溪市白沙乡林业站,浙江,兰溪,321117 |
| 摘 要： | 以石蒜的鳞茎为材料，采用不同的激素配对离体小鳞茎的分化和增殖效果进行了试验研究，结果表明，添加30g/l蔗糖。0.7%琼脂的MS BA（3mg/l) NAA(3mg/l)培养基对小鳞茎的分化效果最佳，而BA（3mg/l) NAA（1mg/l)的激素配比更有利于小鳞茎的增殖，试验中发现，蔗糖的浓度对小鳞茎的增殖与生长影响显著，以60g/l的浓度为最佳，将组织培养获得的小鳞茎切分成块后培养，可获得更多的小鳞茎，BA（0.5-1) NAA(0.5)的激素配比最有利于鳞茎切块增殖；当蔗糖浓度为40g/l时，鳞茎切块结成鳞茎的平均直径达到最大。 |
| 关 键 词： | 石蒜 鳞茎 组织培养 快繁技术 培养基 |
| 文章编号： | 1001-3776(2002)04-0045-04 |
| 修稿时间： | 2002年5月28日 |
Study on Tissue Culture Propagation Technique of Lycoris radiata |
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| ZHU Jin,ZHUGE Qiang,YU Shui-sheng,CHEN Ning-jun.Study on Tissue Culture Propagation Technique of Lycoris radiata[J].Journal of Zhejiang Forestry Science and Technology,2002,22(4):45-48. | |
| Authors: | ZHU Jin ZHUGE Qiang YU Shui-sheng CHEN Ning-jun |
| Institution: | ZHU Jin1,ZHUGE Qiang1,YU Shui-sheng2,CHEN Ning-jun3 |
| Abstract: | Experiment on effect of different hormone concentration on differentiation and multiplication of isolated Ly-coris radiata bulb showed that medium culture of MS+BA 3mg/l+NAA 3mg/l with 30 g/l cane sugar, 0.7% agar had the best effect on differentiation, while BA 3mg/l+NAA 1mg/l had better effect on multiplication. The result indicated that cane sugar concentration had significant effect on multiplication and growth of bulb with the best of 60 g/l. More bulb would be obtained from the tissue cultured one stem. BA 0.5-1+NAA 0.5 had the best effect on multiplication of bulb and mean diameter of stem could be the largest when cane sugar concentration reached 40 g/l. |
| Keywords: | Lycoris radiata bulb multiplication cane sugar |
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