| 大豆“南农86-4”突变体筛选及突变体库的构建 |
| |
| 引用本文： | 韩锁义,张恒友,杨玛丽,赵团结,盖钧镒,喻德跃.大豆“南农86-4”突变体筛选及突变体库的构建[J].作物学报,2007,33(12):2059-2062. |
| |
| 作者姓名： | 韩锁义 张恒友 杨玛丽 赵团结 盖钧镒 喻德跃 |
| |
| 作者单位： | 南京农业大学大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点实验室，江苏南京210095 |
| |
| 基金项目： | 国家重点基础研究发展计划(973计划);国际原子能机构资助项目 |
| |
| 摘 要： | 应用60Co γ射线和甲基磺酸乙酯(EMS)分别对“南农86-4”大豆种子进行诱变，并构建大豆突变体库。结果在M3代分别获得40份和145份叶、茎、花、种子、子叶等性状变异的材料。在两种方式诱变的M3代都获得蛋白质和油含量变化的材料，尤其在EMS诱变的M3代中获得47份蛋白质含量比对照高5个百分点以上的材料，5份蛋白质和油总含量比对照高5个百分点以上的材料。这些突变体可以作为新的种质资源，构建的突变体库也有助于大豆功能基因组研究的开展。
|
| 关 键 词： | 大豆 60Co γ射线 EMS 突变体 |
| 收稿时间： | 2007-04-22 |
| 修稿时间： | 2007-06-21 |
Screening of Mutants and Construction of Mutant Population in Soybean "Nannong 86-4" |
| |
| HAN Suo-Yi,ZHANG Heng-You,YANG Ma-Li,ZHAO Tuan-Jie,GAI Jun-Yi,YU De-Yue.Screening of Mutants and Construction of Mutant Population in Soybean "Nannong 86-4"[J].Acta Agronomica Sinica,2007,33(12):2059-2062. |
| |
| Authors: | HAN Suo-Yi ZHANG Heng-You YANG Ma-Li ZHAO Tuan-Jie GAI Jun-Yi YU De-Yue |
| |
| Institution: | Soybean Research Institute, Nanjing Agricultural University, National Center for Soybean Improvemente, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, Jiangsu, China |
| |
| Abstract: | Soybean mutant population was constructed by treating the seeds of soybean “Nannong 86-4” with 60Co γ rays and EMS respectively. In M3 generations, 40 mutants of leaf, stalk, flower, seed and cotyledon were obtained by treatment of 60Co γ rays, and 145 mutants by EMS. In addition, the mutants of protein and oil contents were obtained; there were 47 mutants with more than 5 percentages on protein content and 5 mutants with more than 5 percentages on total protein and oil content compared with control. These mutants will serve as new germplasm in breeding. The mutant population will be useful to study functional genomics in soybean. |
| |
| Keywords: | EMS |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《作物学报》浏览原始摘要信息 |
| 点击此处可从《作物学报》下载免费的PDF全文 |
|
