| 哈茨木霉Thi4基因的克隆与序列分析 |
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| 引用本文： | 黄彩红,杨谦,刘志华. 哈茨木霉Thi4基因的克隆与序列分析[J]. 北方园艺, 2009, 0(1) |
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| 作者姓名： | 黄彩红 杨谦 刘志华 |
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| 作者单位： | 啥尔滨工业大学生命科学与工程系,黑龙江哈尔滨,150001 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，黑龙江省自然科学基金，国家科技支撑计划重点项目 |
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| 摘 要： | 哈茨木霉(Trichoderma harzianum)是一种丝状土壤真菌,作为一种优秀的生防因子在植物病害防治过程中发挥着重要的作用.为研究其生防机制并获得生防相关基因,构建了菌丝生长期cDNA文库,通过对部分ESTs序列的测定与生物信息学分析,成功获得了噻唑生物合成酶基因Thi4的全长cDNA序列.Thi4基因全长1311bp,包含一个编码322个氨基酸残基长度为969bp的开放读码框,编码蛋白理论分子量为34.5kDa,等电点为5.18.基因的氨基酸序列含有多个功能性位点且在进化上保守.将基因提交国际权威基因数据库GenBank,序列号为DQ647956.
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| 关 键 词： | 哈茨木霉 噻唑生物合成酶 克隆 序列分析 |
Cloning and Sequence Analysis of Gene Thi4 From Trichoderma harzianum |
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| HUANG Cai-hong,YANG Qiao,LIU Zhi-hua. Cloning and Sequence Analysis of Gene Thi4 From Trichoderma harzianum[J]. Northern Horticulture, 2009, 0(1) |
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| Authors: | HUANG Cai-hong YANG Qiao LIU Zhi-hua |
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| Affiliation: | Department of Life Science and Engineering;Harbin Institute of Technology;Harbin;Heilongjiang 150001;China |
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| Abstract: | Trichoderma harzianum is a filamentous soil fungus known as an effective biocontrol agent for a wide range of economically important airborne and soilborne plant pathogens.To explore the integrated bio-control mechanism of T.harzianum and acquire some bio-control associated novel genes,a T.harzianum cDNA library has been constructed and thereby randomly selected clones were sequenced and analyzed by bioinformatics analysis.The full-length cDNA,encoding thiazole biosynthetic enzyme(THI4) with 1318bp nucletid... |
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| Keywords: | Trichoderma harzianum Thiazole biosynthetic enzyme Cloning Sequence analysis |
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