| RAPD技术在茄子种子鉴定中的应用研究 |
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| 引用本文： | 向春阳,王丽君,田秀平,崔丽雅,董炳友. RAPD技术在茄子种子鉴定中的应用研究[J]. 黑龙江八一农垦大学学报, 2003, 15(2): 1-3 |
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| 作者姓名： | 向春阳 王丽君 田秀平 崔丽雅 董炳友 |
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| 作者单位： | 黑龙江八一农垦大学植物科技学院,密山,158308;黑龙江省鸡西市农业广播电视学校 |
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| 基金项目： | 黑龙江农垦总局科委科研资助项目(HKK95-03-04-01-16). |
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| 摘 要： | 以龙杂茄2号、佳杂茄1号2个茄子杂种及其父母本为材料,使用MJ/PTC-200型PCR仪并对电泳图谱分析, 建立了适合茄子随机扩增多态性DNA分析的PCR扩增反应的体系,从40个随机引物中找到了2个可用于鉴定龙杂茄2号、3个可用于鉴定佳杂茄1号杂种纯度的引物.
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| 关 键 词： | 茄子 纯度 鉴定 随机扩增多态性DNA 电泳图谱 |
| 文章编号： | 1002-2090(2003)02-0001-03 |
| 修稿时间： | 2003-03-11 |
Study on Determination of Eggplant Seed by Randomly Amplified Polymorphic DNA Technique |
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| XIANG Chun-yang,WANG Li-jun,TIAN Xiu-ping et al.. Study on Determination of Eggplant Seed by Randomly Amplified Polymorphic DNA Technique[J]. journal of heilongjiang bayi agricultural university, 2003, 15(2): 1-3 |
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| Authors: | XIANG Chun-yang WANG Li-jun TIAN Xiu-ping et al. |
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| Abstract: | PCR experimental system which is suitable for random amplified polymorphic DNA(RAPD) analysis of eggplant was developed by PCR(MJ/PTC-200) and electrophoregrams were analyzed, and two eggplant hybrids (Longzaqie No.2, Jiazaqie No.1 and their parents) were adopted in this experiment. Of the 40 arbitrary primers used in the experiment, 2 primers for Longzaqie No.2 and 3 primers for Jiazaqie No.1 which can determine the purity of hybrid eggplant were obtained. |
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| Keywords: | eggplant purity certification RAPD electrophoregrams |
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