重离子辐照创建水稻直链淀粉、蛋白质突变系的研究

利用重离子辐照‘武运粳7号’创建农艺性状突变体库，用化学方法筛选直链淀粉、蛋白质突变材料，并研究籽粒品质性状与农艺性状的相关性。研究结果显示：202份‘武运粳7号’突变体的直链淀粉含量变幅范围是11.50%~21.70%，其中高含量直链淀粉突变体材料有23份，低含量直链淀粉突变体材料有29份；蛋白质含量变幅范围是7.91%~13.97%，其中高蛋白突变体有29份，低蛋白突变体有24份。进一步在品质性状与农艺性状之间进行相关性分析和主成分分析，相关性分析结果表明：蛋白质含量和每穗实粒数、株高、结实率、千粒重、穗长都呈极显著负相关，相关系数分别为-0.540、-0.593、-0.387、-0.581、-0.563；直链淀粉含量和单穗长呈显著正相关，相关系数为0.294。在主成分分析中将6个农艺性状综合为3个主成分，累积贡献率达89.118%。‘武运粳7号’直链淀粉、蛋白质突变系的获得可为品质性状相关合成途径以及基因调控研究提供基础材料，也为品质育种提供选择依据。

Establishment of Rice Amylose and Protein Mutant Lines by Heavy Ion Irradiation

Agronomic trait mutant library was established by the method of heavy ion irradiation of ‘Wuyunjing7’, amylose and protein mutant materials were screened and the correlation between grain quality and agronomic traits was analyzed. The results showed that the mutation range of amylose content was 11.50%-21.70% of 202 ‘Wuyunjing7’ mutants, 23 belonged to high amylose content mutants and 29 belonged to low amylose content mutants; the mutation range of protein content was 7.91%-13.97%, 29 high protein content mutants and 24 low protein content mutants were obtained. The results of correlation analysis showed that the protein content had a significantly negative correlation with grain number of per spike, plant height, setting rate, thousand kernel weight and panicle length, the correlation coefficient was -0.540, -0.593, -0.387, -0.581 and -0.563, respectively. A significantly positive correlation was found between the amylose content and the panicle length, and the correlation coefficient was 0.294. The principal component analysis showed that the 6 agronomic traits were integrated into 3 principal components, and the accumulative contribution rate reached 89.118%. The establishment of amylose and protein mutant lines could provide materials for the study of synthesis pathway of quality characters and gene regulation and quality breeding.