| 温州蜜柑萎缩病毒的提纯法研究 |
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| 引用本文： | 周常勇,赵学源,蒋元晖,龚祖埙,吴建华,陈作义.温州蜜柑萎缩病毒的提纯法研究[J].西南农业大学学报,1991(1). |
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| 作者姓名： | 周常勇 赵学源 蒋元晖 龚祖埙 吴建华 陈作义 |
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| 作者单位： | 中国农业科学院柑桔研究所
(周常勇，赵学源，蒋元晖)，中国科学院上海生化研究所
(龚祖埙，吴建华)，中国科学院上海生化研究所(陈作义) |
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| 摘 要： | 本文提出的温州蜜柑萎缩病毒(SDV)提纯法,仅用100～200克病洋酸浆叶,且无需通过蔗糖密度梯度离心,在电镜下亦能看到中量直径约25nm 的球状病毒颗粒,粗提纯制剂OD_(860)/OD_(280)平均值为1.28,OD_(260)/OD_(240)平均值为1.15,初步显示出SDV 特征性紫外吸收曲线,提纯病毒产量约为4.5～11mg/kg病叶。比较证明,0.01M、PH7.0和0.1M、PH6.6的柠檬酸缓冲液以及0.5M、PH7.0的磷酸缓冲液是适宜的抽提缓冲液,洋酸浆是最好的繁殖寄主。
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| 关 键 词： | 柑桔属 萎缩病 病毒 提纯方法 |
STUDIES ON PURIFICATION TECHNIQUES FOR SATSUMA DWARF VIRUS |
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| Zhou Changyong Zhao Xueyuan Jiang Yuanhui.STUDIES ON PURIFICATION TECHNIQUES FOR SATSUMA DWARF VIRUS[J].Journal of Southwest Agricultural University,1991(1). |
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| Authors: | Zhou Changyong Zhao Xueyuan Jiang Yuanhui |
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| Abstract: | An improved purification procedure for satsuma dwarf virus (SDV)without sucrose density-gradient centrifugation was developed,by whichglobular SDV particles about 25 nm in diameted were observed with elec-tron microscopy,using only 100-200 g of Physalis floridana-infectedleaves as the virus source.The purufied SDV showed that the ratios ofOD260/OD280 and OD260/OD240 were 1.28 and 1.15,respectively.Theyield of purified SDV ranged from 4.5 to 11 mg/kg infected leaves.Citrate buffer(0.01M,pH7.0 or 0.1M,pH6.6)and phosphate buffer(0.5M,pH7.0) were shown to be preferable for SDV purification and P.floridanais a good propagative host for SDV. |
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| Keywords: | citrus dwarf virus purification methods |
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