Physiological aspects and ileal gene expression profile of early-weaned piglets fed a low threonine diet |
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Authors: | A. Hamard, D. Mazurais, G. Boudry, I. Le Huë rou-Luron, B. S ve,N. Le Floc'h |
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Affiliation: | aINRA, UMR SENAH, 35590 Saint Gilles, France |
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Abstract: | High dietary threonine extraction by the digestive tract suggests that threonine contributes to maintain gut physiology. The aims of this study were to investigate both the physiological disturbances and the molecular events caused by an inadequate dietary threonine supply to the ileum of piglets. We compared paracellular permeability and gene expression, using porcine cDNA microarrays, of the ileum of piglets weaned at 7 days of age fed either a low (LT; n = 7) or a control (C; n = 7) well balanced threonine diet for 2 weeks. At slaughter, the ileal paracellular permeability, measured by FD4 flux in Ussing chambers, increased in piglets fed a LT diet compared with C piglets (P = 0.017). Furthermore, 170 genes had their expression significantly modified in the ileum of the LT piglets: 64 were down regulated and 106 were up regulated. These genes are involved in immune response, apoptosis, energy metabolism, cell migration, epithelial permeability, RNA processing or protein translation. In conclusion, dietary threonine deficiency induced alteration of the ileal barrier and the ileum seems to respond by increasing its metabolic activity. |
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Keywords: | Threonine Ileum Paracellular permeability cDNA microarray Piglets |
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