| 大葱茎尖脱毒苗培养及增殖技术的研究 |
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| 引用本文： | 陈典,黄晓梅,姜玉东,梁艳.大葱茎尖脱毒苗培养及增殖技术的研究[J].园艺学报,2004,31(5):673-675. |
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| 作者姓名： | 陈典 黄晓梅 姜玉东 梁艳 |
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| 作者单位： | (东北农业大学园艺学院, 哈尔滨150030) |
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| 摘 要： | 章丘大葱茎尖外植体启动培养基以MS + NAA 0.1 mg·L-1 + 2iP 0.4 mg·L-1最佳, 诱导率为100 % , 生根壮苗最佳培养基为1/ 2 MS + NAA 0.01 mg·L-1 + IBA 1.5 mg·L-1, 生根率100 %。驯化移栽成活率100 %。分化培养基以MS + NAA 0.1 mg·L-1 + 2iP 0.6 mg·L-1最佳, 丛生率100 % , 平均分化苗数6.8 。根尖染色体观察, 遗传表现稳定。
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| 关 键 词： | 大葱 茎尖培养 增殖 驯化 |
| 文章编号： | 0513-353X(2004)05-0673-03 |
| 收稿时间： | 2004-1-2 |
| 修稿时间： | 2004-5-25 |
Studies on Culture and Propagation of Virus Elimination Seedings from Welsh Onion Shoot-tip |
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| Chen Dian,Huang Xiaomei,Jiang Yudong,and Liang Yan.Studies on Culture and Propagation of Virus Elimination Seedings from Welsh Onion Shoot-tip[J].Acta Horticulturae Sinica,2004,31(5):673-675. |
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| Authors: | Chen Dian Huang Xiaomei Jiang Yudong and Liang Yan |
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| Institution: | ( Department of Horticulture , Northeast Agriculture Universit , Harbin 150030 , China) |
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| Abstract: | The results of virus elimination by shoot-tip culture of welsh onion commonly grown in Zhangqiu showed that the optimum medium for starting culture of shoot-tip was MS + NAA 0.1 mg·L-1 + 2iP 0.4 mg·L-1 , the rate of shoot induction was 100 %; the optimum medium for root induction was 1/ 2 MS + NAA 0.01 mg·L-1 + IBA 1.5 mg·L-1 , both rates of rooting were 100 % , and survival rate after habituation were 100 %。The optimum medium for shoot-tip to differentiate rank seedlings was MS + NAA 0.1 mg·L-1+ 2iP 0.6 mg·L-1 , and the average divided seedings number of each shoot-tip was 6.8 。The observation of cytology showed that this approach could maintain the stability of hereditary. |
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| Keywords: | Welsh onion Shoot-tip culture Propagation Habituation |
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