| 前体饲喂对细胞培养生产紫杉烷的促进作用 |
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| 引用本文： | 周忠强,梅兴国.前体饲喂对细胞培养生产紫杉烷的促进作用[J].林产化学与工业,2005,25(3):51-54. |
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| 作者姓名： | 周忠强 梅兴国 |
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| 作者单位： | 中南民族大学,化学与材料科学学院,湖北,武汉,430074;军事医学科学院,毒物药物研究所,北京,100850 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，中南民族大学校科研和教改项目 |
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| 摘 要： | 研究了前体化合物对中国红豆杉细胞培养生产紫杉烷的影响.0.5 mmol/L 丙酮酸钠使紫杉醇和1β-羟基巴卡亭Ⅰ的产率分别增加236 %和225.8 %.2 mmol/L 3-甲基 -2-丁烯 -1-醇和0.4 mmol/L苯甲酸钠使2α,5α,10β,14β-四乙酰氧基 -紫杉 -4(20),11-二烯产率分别提高218.6 %和245.2 %.1 mmol/L乙酸钠和0.5 mmol/L 丙酮酸钠使14β-2-甲基丁酰氧基 -2α,5α,10β-三乙酰氧基-紫杉 -4(20),11-二烯的产率分别提高193 %和210.4 %.结果表明添加适当浓度的前体有利于紫杉烷的合成.
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| 关 键 词： | 紫杉烷 细胞悬浮培养 前体 |
| 文章编号： | 0253-2417（2005）03-0051-04 |
| 收稿时间： | 06 7 2004 12:00AM |
| 修稿时间： | 2004-06-07 |
ENHANCEMENT OF TAXANES PRODUCTION IN CELL SUSPENSION CULTURES OF TAXUS CHINENSIS BY PRECURSOR-FEEDING |
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| ZHOU Zhong-qiang,MEI Xing-guo.ENHANCEMENT OF TAXANES PRODUCTION IN CELL SUSPENSION CULTURES OF TAXUS CHINENSIS BY PRECURSOR-FEEDING[J].Chemistry & Industry of Forest Products,2005,25(3):51-54. |
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| Authors: | ZHOU Zhong-qiang MEI Xing-guo |
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| Abstract: | The effects of precursors on taxanes production in cell suspension cultures of Taxus chinensis(Pilg.)Rehd. were studied. Feeding of sodium pyruvate at 0.5 mmol/L enhanced the yields of taxol and 1β-hydroxy baccatin Ⅰat 336 % and 325.8 % of control, respectively. Taxuyunnanine C yield was greatly promoted in presence of 3-methyl-2-buten-1-ol at 2 mmol/L (318.6 % of control) and sodium benzoate at 0.4 mmol/L (345.2 % of control). Yield increase of 14β-(2-methyl-butyryl)oxo-2α,5α,10β-triacetoxytaxa-4(20), 11-diene was most pronounced in presence of sodium acetate (293 % of control at 1 mmol/L) and sodium pyruvate (310.4% of control at 0.5 mmol/L). The results showed that precursor-feeding at optimal concentration is advantageous to biosynthesis of taxanes. |
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| Keywords: | taxanes cell suspension culture precursor |
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