大约克猪和二花脸猪SLA-DRB基因外显子2的PCR-RFLP多态性分析

采用PCR-RFLP方法分析大约克猪和二花脸猪SLA-DRB外显子2的多态性.结果显示:Rsa Ⅰ酶切后得到3种等位基因条带,分别为A(141/93/11 bp)、B(111/69/54/11 bp)、D(93/48/39/54/11 bp).χ2检测表明:二花脸猪SLA-DRB基因外显子2处于Hardy-Weinberg平衡状态(P>005),而大约克猪处于非平衡状态(P<001).两品种A等位基因均为优势等位基因,AA型为主要基因型,其频率在品种间无显著差异.首次发现等位基因B、D分别出现在二花脸猪和大约克猪中,有望用于区分两品种.

Analysis of polymorphism on exon 2 of SLA-DRB in Large Yorkshire and Erhualian pigs with PCR-RFLP

The exon 2 of SLA-DRB gene in Large Yorkshire and Erhualian pigs was analyzed with PCR-RFLP. The results showed which after digested by restriction endonuclease Rsa Ⅰ , three kinds of band patterns were created, which were A; 141/93/11 bp, B: 111/69/54/11 bp and D: 93/48/39/54/11 bp. The frequencies of all pattern types were Hardy-Weinberg Equilibrium in Erhualian populations, while the same results did not appear in Large Yorkshire pigs. In each breed, A was the dominant band pattern and AA was the dominant genotype. There were no significant differences in the frequency of A band pattern among these two breeds. In these two breeds, B was only in Erhualian pigs, and D was only in Large Yorkshire pigs. The result suggested that B and D could be used to distinguish the two breeds and that benefited molecular breeding.