乐果液相色谱与气相色谱检测方法的比较

摘 要：【研究目的】通过应用液相色谱法（HPLC）和气相色谱法（GC）对乐果测定的参数进行比较，为乐果检测方法的选择提供参考。【方法】分别应用气相色谱和高效液相色谱法对乐果检测条件进行优化，对这两种测定方法的灵敏度、线性相关性、稳定性及添加回收率的等参数测定结果进行比较。【结果】用液相色谱法测定乐果最低检测限为0.01mg/L，比用气相色谱法（最低检测到0.1mg/L）测定时有更高的灵敏度；HPLC检测（r＝0.9990）较GC（r＝0.9937）有更好的线形相关性；在乐果浓度低于0.1mg/L时，用液相色谱法对乐果进行测定有很好的稳定性，在浓度高于0.1mg/L，两种测定方法的稳定性相当。【结论】用液相色谱法测定乐果完全可以达到农药登记残留试验准则的要求，它比气相色谱法更适合乐果的微量及常量的检测。

Comparison between the Detection Methods of Dimethoate by HPLC and GC

Abstract: 【OBJECT】Comparison between parameters in Dimethoates detection tests by using HPLC and GC was conducted, in order to supply reference for selection of Dimethoates detection method. 【METHOD】 The detection conditions of Dimethoates by using HPLC and GC were optimized respectively, and then the sensitivity, linear relevancy, stability and adding recovery rate of these two method were compared. 【RESULTS】Comparing with the lowest detection limit of 0.1mg/L for Dimethoates by GC, higher sensitivity of HPLC with 0.01mg/L was recorded. Also, HPLC had better linear relevancy than GC. When the concentration of Dimethoates lower than 1.0mg/L, better stability was found by HPLC than GC; but the similar stabilities were observed while the concentration higher than 0.1mg/L. 【CONCLUSION】HPLC was better than GC as the macro- and micro-detections for Dimethoates, which could achieve the requirement of Pesticide Register Residue Test Rule in China.