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树莓组培快繁技术体系研究
引用本文:张林水,李志民,李波,吴霞,上官小霞,李燕娥. 树莓组培快繁技术体系研究[J]. 山西农业科学, 2006, 34(1): 32-34
作者姓名:张林水  李志民  李波  吴霞  上官小霞  李燕娥
作者单位:山西省农业科学院棉花研究所,山西,运城,044000;山西省绿洲科技有限公司,山西,运城,044000
摘    要:树莓外植体用HgCl2消毒时间以10min为宜,培养基MS附加6-BA0.5mg/L+NAA0.20~0.25mg/L,每株增殖9.2~9.8个;培养基附加IBA0.2mg/L,每株平均生根数3.09条;树莓试管苗生根4~6条及根长1.0cm以上,扣杯保湿时间超过4周,避开高温干燥的季节移栽,成活率为79.6%~84.6%。

关 键 词:树莓  组培  快繁  移栽  成活率
文章编号:1002-2481(2006)01-0032-03
收稿时间:2004-09-13
修稿时间:2004-09-13

Rapid-Propagation Technique System of Rubus Crataegifolius through Tissues Culture
ZHANG Lin-shui,LI Zhi-ming,LI Bo,WU Xia,SHANGGUANG Xiao-xia,LI Yan-e. Rapid-Propagation Technique System of Rubus Crataegifolius through Tissues Culture[J]. Journal of Shanxi Agricultural Sciences, 2006, 34(1): 32-34
Authors:ZHANG Lin-shui  LI Zhi-ming  LI Bo  WU Xia  SHANGGUANG Xiao-xia  LI Yan-e
Affiliation:1. Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China ; 2.Luzhou Science and Technology Limited Company in Shanxi, Yuncheng 044000, China
Abstract:The proper sterilized time for the explants of Rubus crataegifoliuswas 10 minutes in Hgcl2. The fruit numbers could be increased by 9.2~9.8/plant by means of media supplemented with 6-BA 0.5 mg/L + NAA 0.2~0.25mg/L, while the roots could be increased by 3.09 roots/plant in media supplemented with IBA 0.2mg/L. Each tuber seedling had 4~6 roots and each of them was 1.0cm long. After transplanting, the seedlings were covered with plastic cups for over 4 weeks.When transplanting is done during non high temperature and dry seasons,the survival rate was 79.6%~84.6%.
Keywords:Rubus crataegifolius  Tissues culture  Rapid-propagation  Transplant survival rate  
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