A new method for recording activities of spinal motoneurons during fictive swimming
in paralyzed teleost fish |
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Authors: | MASAYUKI YOSHIDA YASUHIKO MUROCHI YOSHIHIKO BABA AND KAZUMASA UEMATSU |
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Institution: | Laboratory of Fish Physiology, Faculty of Applied Biological Science, Hiroshima University, Higashi-hiroshima, Hiroshima 739-8528, Japan |
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Abstract: | ABSTRACT: A new method for monitoring spinal motoneuron activities during fictive swimming in teleost fish was developed. Enamel-insulated copper wire electrodes were implanted in the trunk muscle of goldfish or carp. For each freely moving fish, an electromyogram (EMG) was recorded using the electrodes. In those fish paralyzed with curare, using the same electrode set, bursts of electrical activities consisted of spikes of smaller amplitude and of shorter duration compared with those recorded by EMG. Simultaneous recording of the extracellular activity and intracellular recording from the muscle revealed that the bursts of spikes recorded in the paralyzed fish were motor nerve impulses innervating the muscle and are considered to be fictive swimming activity. The method developed in the present study provides a useful tool with which to investigate neuronal mechanisms underlying swimming activity in teleost fish. |
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Keywords: | brain carp fictive swimming goldfish wire electrode |
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