| 蚯蚓纤溶酶的分离纯化工艺研究 |
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| 引用本文： | 陈安和 茹炳根. 蚯蚓纤溶酶的分离纯化工艺研究[J]. 西南大学学报(自然科学版), 1994, 16(4): 400-402 |
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| 作者姓名： | 陈安和 茹炳根 |
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| 作者单位： | 西南师范大学生物系，北京大学生物系 重庆 630715，北京 100871 |
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| 基金项目： | 重庆市科委重点科研项目 |
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| 摘 要： | 对人工饲养的北星二号蚯蚓纤溶酶的分离纯化表明，其较佳工艺为磷酸缓冲液保温或匀浆提取，Ａｍｂｅｒｌｉｔｅ ＩＲＡ－９０４或ＤＥＡＥ－纤维素柱层析，Ｓｅｐｈａｄｅｓ Ｇ－１００凝胶过滤，真空冷冻干燥，按此工艺可得到纤溶化活力为８３７００ｍｍ＾２／ｍｇ。蛋白的蚯蚓纤溶酶。
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| 关 键 词： | 蚯蚓 酶 分离 提纯 纤溶酶 |
STUDIES ON THE TECHNOLOGY FOR SEPARATION AND PURIFICATION OF EARTHWORM FIBRINOLYSIN |
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| Chen Anhe. STUDIES ON THE TECHNOLOGY FOR SEPARATION AND PURIFICATION OF EARTHWORM FIBRINOLYSIN[J]. Journal of southwest university (Natural science edition), 1994, 16(4): 400-402 |
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| Authors: | Chen Anhe |
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| Abstract: | Artificially fed earthworms (Eisenia foetida) were used as experimental material for separating and purifying earthworm fibrinolysin. A comparatively satisfactory technology was developed through screening and comparing Id kinds of ion exchange resins and several kinds of DEAE -cellulose. Its main steps are as follows; pounding the fresh earthworm into small pieces, homogenizing them before extraction of incubating them with phosphate buffer solution, separating by ion exchange chromatography with Amberlite IRA - 904 or DEAE - cellulose, filting with Sephadex G- 100 gel and vacuum freeze -drying. Requiring only simple equipment and easy and convenient to manipulate, this technology is suitable for large - scale production of earthworm fibrinolysin. |
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| Keywords: | earthworm enzyme separation purification |
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