除虫菊愈伤组织和发状根中除虫菊酯杀虫活性测定

以除虫菊作为材料探讨除虫菊愈伤组织和发状根中除虫菊酯的杀虫活性，为利用细胞工程生产除虫菊酯提供理论依据。以除虫菊的茎和叶作为外植体诱导愈伤组织和发状根，用丙酮浸提愈伤组织和发状根中的除虫菊酯，然后以粘虫作为测试对象采用浸虫和浸叶2 种方式测定除虫菊酯的杀虫活性。结果表明：除虫菊愈伤组织和发状根中的除虫菊酯有杀虫活性，其对粘虫的拒食作用强于触杀作用，且发状根中除虫菊酯的杀虫活性优于愈伤组织，以浸虫方式处理的除虫菊酯杀虫活性更高。本文为未来利用细胞工程方式工业化生产除虫菊酯开拓了新途径。

Insecticidal Activity of Pyrethrin in Hairy Roots and Callus Induced from Pyrethrum cinerariifolium Trev.

Pyrethrum cinerariifolium Trev. was used as the material to detect insecticidal activity of pyrethrin from hairy roots and callus so as to supply certain theoretical basis for producing pyrethrin by cell engineering. The stems and leaves of Pyrethrum cinerariifolium were taken as explants to induce the callus and hairy roots. Acetone was used to extract pyrethrin and armyworm was selected to test the insecticidal activity by armyworm dipping and leaf dipping method. The results showed that pyrethrin extracted from the callus and hairy roots had insecticidal activity. Its antifeedant activity was better than contact toxicity. The insecticidal activity of pyrethrin from hairy roots was better than that from callus. And the insecticidal activity tested by armyworm dipping method was better than that of leaf dipping method. This study provides a new way for the future industrial production of pyrethrin by cell engineering.