| 气相色谱法速测叶用莴苣的乙烯释放量 |
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| 引用本文： | 及雪良,刘雪莹,张喜春,杨 柳,刘中华,韩莹琰.气相色谱法速测叶用莴苣的乙烯释放量[J].中国农学通报,2016,32(22):63-67. |
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| 作者姓名： | 及雪良 刘雪莹 张喜春 杨 柳 刘中华 韩莹琰 |
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| 作者单位： | (;1.北京农学院植物科学技术学院/北京市农业应用新技术重点实验室,北京 102206；;2.北京市优质农产品产销服务站,北京 100101） |
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| 基金项目： | 国家自然科学基金“叶用莴苣高温胁迫响应因子LsHsp70的功能研究”(31401883)；北京市教育委员会市属高校创新能力提升项目“高产优质生菜产业化关键技术示范与成果转化(PXM2016_014207_000010)；北京市支农资金农业科技项目“生菜耐热新品种繁育及安全生产技术试验示范”(20140134)。 |
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| 摘 要： | 针对叶用莴苣（生菜）高温胁迫下易抽薹的生理特性，为探究内源乙烯含量在叶用莴苣抽薹过程中所起的作用，采用气相色谱法测定乙烯的释放量，比较不同时间、取样部位、温度、光照以及水分条件对乙烯释放量的影响。研究结果表明，叶用莴苣的最佳取样部位为茎，取样时间为6 h，取样温度为30℃。同时我们优化了色谱测定条件， 使得乙烯与空气分离较好，得到了基线稳定的对称峰。
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| 关 键 词： | DNA指纹图谱 DNA指纹图谱 SSR分子标记 水稻 上海 |
| 收稿时间： | 2015/12/25 0:00:00 |
| 修稿时间： | 2016/2/19 0:00:00 |
Determination of Ethylene Release Rate in Lettuce by Gas Chromatography |
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| Ji Xueliang,Liu Xueying,Zhang Xichun,Yang Liu,Liu Zhonghua and Han Yingyan.Determination of Ethylene Release Rate in Lettuce by Gas Chromatography[J].Chinese Agricultural Science Bulletin,2016,32(22):63-67. |
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| Authors: | Ji Xueliang Liu Xueying Zhang Xichun Yang Liu Liu Zhonghua and Han Yingyan |
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| Institution: | (;1.College of Plant Science and Technology, Beijing University of Agriculture/ Beijing Key Laboratory for Agricultural Application and New Technique, Beijing 102206;2.Beijing Production and Marketing Service Station for Superior Agricultural Products, Beijing 100101) |
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| Abstract: | Lettuce is bolting easily under high temperature stress. To explore the role of endogenous ethylene in the process of lettuce bolting, we used the gas chromatography to measure the ethylene release content, compared the effects of different time, sampling parts, temperatures, light and moisture conditions on ethylene release. The results showed that the optimal sampling part was stem, the time was 6 h and the temperature was 30℃. We also optimized the chromatographic condition at the same time, made ethylene and air separate better, and got the symmetrical peak of stable baseline. |
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| Keywords: | lettuce ethylene gas chromatography |
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