| L-丝氨酸产生菌c-11中serA基因的克隆与表达 |
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| 引用本文： | 谢玉清,罗淑萍,茆军.L-丝氨酸产生菌c-11中serA基因的克隆与表达[J].新疆农业科学,2007,44(5):652-653. |
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| 作者姓名： | 谢玉清 罗淑萍 茆军 |
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| 作者单位： | 新疆农业大学农学院,乌鲁木齐,830052;新疆农科院微生物所,乌鲁木齐,830091 |
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| 摘 要： | 利用PCR技术以L-丝氨酸产生菌株黄色短杆菌c-11的染色体DNA为摸板,扩增出serA基因,连接到表达载体pEC7上,转化L-丝氨酸产生菌c-11,使其氨基酸产量由12 g/L增加到18 g/L,产酸率增加了50;.
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| 关 键 词： | 黄色短杆菌C-11 serA基因 克隆 表达 |
| 文章编号： | 1001-4330（2007）05-0652-02 |
| 收稿时间： | 2006-11-20 |
| 修稿时间： | 2007-01-29 |
Cloning and Expression of SerA in C-11 Producing L-serine |
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| XIE Yu-qing,LUO Shu-ping,Mao Jun.Cloning and Expression of SerA in C-11 Producing L-serine[J].Xinjiang Agricultural Sciences,2007,44(5):652-653. |
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| Authors: | XIE Yu-qing LUO Shu-ping Mao Jun |
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| Institution: | 1. College of Agronomy, Xinjiang Agricultural University, Urumqi 830091, China ; 2. Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830052, China |
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| Abstract: | Cloning serA from Brevibacterium flavm C-11 was amplified by PCR and was linked to vector pEC7,then serA in c-11 producing L-serine was transformed to enhance production of serine from 12 g/L to 18 g/L produced by C-11. |
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| Keywords: | Brevibacterium flavm C-11 serA cloning expression |
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