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| 高效液相色谱法测定罗非鱼鱼苗和不同来源罗非鱼中甲基睾丸酮残留 | |
| 引用本文： | 洪二利,郑光明,吴仕辉,孟娣,谢文平,宋怿,马兵,卢迈新,戴晓欣,马丽莎.高效液相色谱法测定罗非鱼鱼苗和不同来源罗非鱼中甲基睾丸酮残留[J].广东农业科学,2011,38(10):138-141. |
| 作者姓名： | 洪二利 郑光明 吴仕辉 孟娣 谢文平 宋怿 马兵 卢迈新 戴晓欣 马丽莎 |
| 作者单位： | 1. 上海海洋大学水产与生命科学学院,上海200090;中国水产科学研究院珠江水产研究所,广东广州510380 2. 中国水产科学研究院珠江水产研究所,广东广州,510380 3. 中国水产科学研究院质量与标准研究中心,北京,100141 |
| 基金项目： | 中国水产科学研究院院部中央级公益性科研院所基本科研业务费专项(2008C018) |
| 摘 要： | 用高效液相色谱法(HPLC)法对投喂添加不同浓度甲基睾丸酮(MET)饲料的罗非鱼鱼苗、随机采取的罗非鱼成鱼及其跟踪饲料进行检测。刚孵化的罗非鱼鱼苗投喂添加不同浓度MET饲料30 d后,HPLC检测其体内残留量。继续投喂未添加药物的饲料,30 d后再次检测MET残留量。分别从不同的水产品批发市场、农贸市场和罗非鱼主要养殖产地,随机抽取罗非鱼成鱼样品,并从养殖产地随机抽取追踪饲料样品,高效液相色谱法检测MET残留。结果表明,投喂30 d后的罗非鱼苗种,对照组无MET残留,其余5个添加MET试验组的MET残留量随着添加浓度的增加而增加;继续投喂未添加MET的正常饲料30 d后,所有试验组均无MET残留;从各批发市场、农贸市场以及各个养殖基地随机抽检的罗非鱼样品及其跟踪饲料中MET阳性率为零。 |
| 关 键 词： | 罗非鱼 高效液相色谱 甲基睾丸酮残留 罗非鱼鱼苗 饲料 |
Determination of methyltestosterone residue of tilapia fry and tilapia from different sources using high performance liquid chromatography |
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| HONG Er-li,ZHENG Guang-ming,WU Shi-hui,MENG Di,XIE Wen-ping,SONG Yi,MA Bing,LU Mai-xin,DAI Xiao-xin,MA Li-sha.Determination of methyltestosterone residue of tilapia fry and tilapia from different sources using high performance liquid chromatography[J].Guangdong Agricultural Sciences,2011,38(10):138-141. | |
| Authors: | HONG Er-li ZHENG Guang-ming WU Shi-hui MENG Di XIE Wen-ping SONG Yi MA Bing LU Mai-xin DAI Xiao-xin MA Li-sha |
| Institution: | HONG Er-li1,2,ZHENG Guang-ming1,WU Shi-hui1,MENG Di3,XIE Wen-ping1,SONG Yi3,MA Bing3,LU Mai-xin1,DAI Xiao-xin1,MA Li-sha1 (1.Pearl River Fishery Research Institute,Chinese Academic of Fishery Science,Guangzhou 510380,China,2.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 200090,3.Quality and Standard Center,Beijing 100141,China) |
| Abstract: | High performance liquid chromatography(HPLC)method was used to detect the tilapia fries which was fed feed adding with different concentrations of methyltestosterone(MET) and adult tilapias which were taken randomly and the tracking feed of adult tilapias.The newly hatched tilapia fries were fed with feed adding different concentration of MET.After feeding for 30 days,the body MET residue was detected using HPLC method,and then continuing to feed feed without MET,30 days later,detected MET residues again.Fr... |
| Keywords: | high performance liquid chromatography(HPLC) methyltestosterone residues tilapia fry tilapia feed |
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