| 内生放线菌A-1对苹果果实轮纹病的防效及防御性酶活性的影响 |
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| 引用本文： | 雍道敬,王彩霞,李桂舫,李保华.内生放线菌A-1对苹果果实轮纹病的防效及防御性酶活性的影响[J].植物保护学报,2014,41(3):335-341. |
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| 作者姓名： | 雍道敬 王彩霞 李桂舫 李保华 |
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| 作者单位： | 青岛农业大学农学与植物保护学院, 山东省植物病虫害综合防控重点实验室, 青岛266109;青岛农业大学农学与植物保护学院, 山东省植物病虫害综合防控重点实验室, 青岛266109;青岛农业大学农学与植物保护学院, 山东省植物病虫害综合防控重点实验室, 青岛266109;青岛农业大学农学与植物保护学院, 山东省植物病虫害综合防控重点实验室, 青岛266109 |
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| 基金项目： | 现代农业产业技术体系（CARS-28），公益性行业（农业）科研专项（nyhyzx3-22），山东省科技攻关计划（2010GNC10918），国家自然科学基金（31000891，31272001），山东省“泰山学者”建设工程 |
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| 摘 要： | 为揭示内生放线菌A-1对苹果果实轮纹病的防效及防病机制，采用平板法和喷雾处理刺伤接种法，测定了其对苹果轮纹病菌的抑制作用及果实内防御性酶活性的影响。结果表明：菌株A-1能显著抑制轮纹病菌菌丝生长，104 CFU/mL菌悬液的抑制率达90%以上；喷施107 CFU/mL A-1菌悬液后，间隔12 h以上接种的各处理，3 d和7 d时防效分别为91.79%~95.67%和77.41%~94.00%，均与对照农药苯醚甲环唑相当。喷施107 CFU/mL A-1菌悬液后接种或不接种轮纹病菌的处理，果实内过氧化物酶、过氧化氢酶、多酚氧化酶和苯丙氨酸解氨酶活性显著升高，且均高于只接种轮纹病菌的处理；喷施A-1菌悬液后接种病原菌的处理酶活性增加最显著，其峰值是对照的2.30~11.00倍。表明内生放线菌A-1可通过产生拮抗物质、提高寄主防御性酶活性等机制实现对苹果轮纹病的有效防治。
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| 关 键 词： | 放线菌A-1 苹果轮纹病菌 防效 防御性酶 生防机制 |
| 收稿时间： | 2013/11/18 0:00:00 |
Control efficiency of endophytic actinomycetes A-1 against apple fruit ring rot and its influence on the activity of defense-related enzymes |
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| Yong Daojing,Wang Caixi,Li Guifang and Li Baohua.Control efficiency of endophytic actinomycetes A-1 against apple fruit ring rot and its influence on the activity of defense-related enzymes[J].Acta Phytophylacica Sinica,2014,41(3):335-341. |
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| Authors: | Yong Daojing Wang Caixi Li Guifang and Li Baohua |
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| Institution: | Key Laboratory of Integrated of Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao266109, Shandong Province, China;Key Laboratory of Integrated of Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao266109, Shandong Province, China;Key Laboratory of Integrated of Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao266109, Shandong Province, China;Key Laboratory of Integrated of Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao266109, Shandong Province, China |
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| Abstract: | In order to reveal the control efficiency and biocontrol mechanism of endophytic actinomycetes strain A-1 against apple fruit ring rot caused by Botryosphaeria dothidea, the inhibitory efficiency of strain A-1 to B.dothidea and its effect on the activities of defense-related enzymes in apple fruits were determined using the agar plate method and spraying strain A-1 combined with wound inoculation. The stain A-1 significantly inhibited the growth of B.dothidea and the inhibitory rate was more than 90% when 104 CFU/mL cell culture was used. When applying 107 CFU/mL cell culture of strain A-1 for more than 12 h, followed by B.dothidea inoculation, the control efficiency was 91.79%-95.67% or 77.41%-94.00% at 3 or 7 d post inoculation, respectively, which was comparable to that of difenoconazole treatment. The activities of peroridase (POD), catalase (CAT), polyphenol oxidase (PPO), and phenylalanine ammonialyase (PAL) were significantly enhanced in apple fruits pre-treated with strain A-1 alone or in combination with B.dothidea inoculation. The enzyme activities increased significantly in fruits treated with 107 CFU/mL cell culture of strain A-1, followed by B.dothidea inoculation. The activity peaks of PPO, CAT, POD and PAL in treatments were 2.30 to 11.00 times higher than those of controls. The endophytic actinomycetes strain A-1 was effective in protecting apple fruit against B.dothidea infection via producing antagonistic substances and increasing the activities of defense-related enzymes. |
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| Keywords: | actinomycetes strain A-1 Botryosphaeria dothidea control efficiency defense-related enzyme biocontrol mechanism |
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