| 大孔树脂在纯化杜仲总黄酮中的应用 |
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| 引用本文： | 刘志祥. 大孔树脂在纯化杜仲总黄酮中的应用[J]. 湖北农业科学, 2009, 48(6) |
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| 作者姓名： | 刘志祥 |
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| 作者单位： | 中南林业科技大学生命科学与技术学院,长沙,410004 |
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| 基金项目： | 中南林业科技大学青年基金,中南林业科技大学林木遗传育种学科资助项目 |
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| 摘 要： | 为确定大孔树脂分离杜仲总黄酮的最佳工艺条件,以吸附率及解吸率为考察指标,确定最佳型号树脂、静态和动态吸附及解吸的相关影响因素.结果表明,D-100型大孔树脂时杜仲总黄酮有良好的吸附性,其吸附分离的工艺条件的药液浓度为0.4mg·mL-1,以2mL·min-1吸附速率进行吸附,5倍柱体积70%乙醇,洗脱速率为1 mL·min-1时洗脱效果最佳.该方法简单易行,分离效果好,适于杜仲总黄酮的分离纯化.
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| 关 键 词： | 大孔树脂 纯化 杜仲 总黄酮 |
Application on the Purification Flavoniods from Eucommia ulmoides by Macroporous Resin |
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| LIU Zhi-xiang. Application on the Purification Flavoniods from Eucommia ulmoides by Macroporous Resin[J]. Hubei Agricultural Sciences, 2009, 48(6) |
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| Authors: | LIU Zhi-xiang |
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| Affiliation: | College of Life Science and Technology;Central South University of Forestry and Technology;Changsha 410004;China |
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| Abstract: | To establish the optimum process conditions for purification of flavoniods from Eucommia ulmoides by macroporous resin,Seven types of macroporous resin were used to purify flavoniods of Eucommia ulmoides,which determined the optimum macroporous resin,relative factors of adsorption and desorption for static state and dynamic state through adsorption and des-orption rate.The results showed that the D-100 macroporous resin had good absorbing effect on flavoniods of Eucommia ulmoides.The absorbing technological... |
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| Keywords: | macroporous resin purification Eucommia ulmoides total flavonoids |
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