芦笋花药离体培养技术研究 |
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引用本文: | 李新江,建德锋,陈刚. 芦笋花药离体培养技术研究[J]. 安徽农业科学, 2010, 38(17): 8861-8862. DOI: 10.3969/j.issn.0517-6611.2010.17.012 |
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作者姓名: | 李新江 建德锋 陈刚 |
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作者单位: | 吉林农业科技学院,吉林吉林,132101;吉林农业科技学院,吉林吉林,132101;吉林农业科技学院,吉林吉林,132101 |
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摘 要: | [目的]提高芦笋的产量。[方法]以不同阶段的芦笋花蕾为外植体,筛选花粉的最佳生长阶段(即最佳材料),研究不同浓度激素配比对芦笋芽分化及生根的影响。[结果]以花粉处于单核期的花蕾为最佳试材,芽分化的最佳培养基为MS+6-BA 2.0 mg/L+NAA1.0 mg/L;最佳生根培养基为MS+KT 0.1 mg/L+NAA 0.20 mg/L。[结论]该试验结果为芦笋的快速繁殖奠定了基础。
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关 键 词: | 芦笋 培养基 外植体 花药 |
Anther Culture of Asparagus officinalis in vitro |
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Affiliation: | LI Xin-jiang et al(Jilin Agricultural Science and Technology University,Jilin,Jinlin 132101) |
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Abstract: | [Objective] The study aimed to enhance the yield of Asparagus officinalis.[Method]With different stage flower bud of Asparagus officinalis as explants,the effects of different concentration of hormone combination on bud differentiation and rooting of Asparagus officinalis were selected.[Result] With bud which pollen in uninucleate stage as the best test materials,the best culture medium of bud differentiation was MS+6-BA 2.0 mg/L+NAA 1.0 mg/L;the best rooting medium was MS+KT 0.1 mg/L+ NAA 0.20 mg/L.[Conclusion]The test results established basis for rapid propagation of Asparagus officinalis. |
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Keywords: | Asparagus officinalis Culture medium Explant Anther |
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