微粒蒙脱石对猪组织铅水平、红细胞生成和肝脏ALA-D酶活性及铅诱导的脂质过氧化的影响

选60头体质量约33kg的“杜长大”杂交猪，随机分成2组，每组3个重复，一组饲以基础日粮＋10mg/kg铅＋0.5％微粒蒙脱石，另一组饲以基础日粮＋10mg／kg铅作为对照。研究结果表明：添加微粒蒙脱石组与对照组相比，明显降低了猪全血、脑、肝、肾、骨和毛等组织中铅含量：通过血细胞计数、血红素和血球容积测定表明，添加微粒蒙脱石组的红细胞生成显著增高，且肝脏中ALA—D酶活性显著提高。铅对肝脏的损伤作用明显。表现为丙二醛（MDA）含量显著升高（17．08％），过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）含量分别降低85．73％、52．17％、47．56％，而在日粮中添加微粒蒙脱石可以显著改善铅诱导的上述损伤。结果提示，微粒蒙脱石对铅中毒有潜在的治疗作用。

Effects of particulate montmorillonite on tissue lead levels, erythropoiesis,ALA-D activity,and lead-induced lipid peroxidation in liver of pigs

Sixty barrows (Duroc × Labdrace × Yorkshine) were randomly assigned to two groups by weight of 33 kg,each of which was replicated three times with ten pigs. Half of the pigs were fed with diets containing 10 mg/kg lead and 0.5% particulate montmorillonite,the other half pigs were fed only with diets supplemented 10 mg/kg lead as control groups for 100 days.The results showed that the addition of particulate montmorillonite to the diet significantly decreased lead concentration in tissues such as blood,brain,liver,bone,kidney and hair and enhanced the erythropoiesis as measured by increasing numbers of RBC,hemoglobin and hematocrit values ,and elevated ALA-D activity in liver. The damage of lead to the liver was evident in the increases in hepatic concentration of malondialdehyde (+ 17.08% );decreases in the antioxidant enzymes catalase(-85.73 % ),superoxide dismutase ( - 52.17% ) and glutathione peroxidase ( - 47.56% ). Concomitant use of particulate montmorillonite in the diets completely ameliorated the lead-induced oxidative damage. It indicated that particulate montmorillonite is possessed of the potential therapeutic activity against lead poison.