| 棉花八氢番茄红素脱氢酶GhPDS1基因的克隆与表达谱分析 |
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| 引用本文： | 徐大伟,张雨良,檀根甲.棉花八氢番茄红素脱氢酶GhPDS1基因的克隆与表达谱分析[J].棉花学报,2011,23(3):200-204. |
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| 作者姓名： | 徐大伟 张雨良 檀根甲 |
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| 作者单位： | 1. 安徽农业大学植物保护学院,合肥,230036
2. 中国热带农业科学院热带生物技术研究所/农业部热带作物生物技术,重点开放实验室,海口,571101 |
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| 基金项目： | 国家转基因生物新品种培育重大专项 |
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| 摘 要： | 八氢番茄红素脱氢酶(PDS)在胡萝卜素生理代谢和病毒诱导外源基因沉默中起着非常重要的作用.本研究通过同源克隆方法,分析已报道植物八氢番茄红素脱氢酶氨基酸保守区,设计筒并引物,扩增出编码棉花PDS基因cDNA中间片段.通过RACE技术成功地克隆了棉花PDS基因的全长cDNA,命名为GhPDS1(GenBank No.HQ...
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| 关 键 词： | 棉花 八氢番茄红素脱氢酶 GhPDS1基因 RACE技术 序列分析 表达谱 |
| 收稿时间： | 2010-11-11; |
Molecular Cloning and Expression Analysis of GhPDS1 Gene in Gossypium hirsutum |
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| XU Da-wei,ZHANG Yu-liang,TAN Gen-jia.Molecular Cloning and Expression Analysis of GhPDS1 Gene in Gossypium hirsutum[J].Cotton Science,2011,23(3):200-204. |
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| Authors: | XU Da-wei ZHANG Yu-liang TAN Gen-jia |
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| Institution: | XU Da-wei1,ZHANG Yu-liang2,TAN Gen-jia1(1.College of Plant Protection,Anhui Agricultural University,Hefei 230036,China,2.Key Laboratory for Tropical Crop Biotechnology,Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology,CATAS,Haikou 571101,China) |
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| Abstract: | Phytoene desaturase(PDS) play an important role in the physiological metabolism of carotene and virus induced silencing of foreign genes.The degenerated primers were designed from the conserved motifs of phytoene desaturase gene isolated from other plants in NCBI,and the middle fragments of PDS gene in Gossypium hirsutum were amplified.The 5'and 3'ends of the cDNA were cloned through RACE methods.The gene of PDS in Gossypium hirsutum was named as GhPDS1(GenBank accession number: HQ441484).The semi-quantitat... |
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| Keywords: | Gossypium hirsutum phytoene desaturase GhPDS1 RACE technology sequence analysis expression profiling |
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