| 不同来源黄瓜遗传亲缘关系的RAPD分析 |
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| 引用本文： | 刘殿林,杨瑞环,哈玉洁,陈德富,陈喜文,夏立新. 不同来源黄瓜遗传亲缘关系的RAPD分析[J]. 华北农学报, 2003, 18(3): 50-52 |
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| 作者姓名： | 刘殿林 杨瑞环 哈玉洁 陈德富 陈喜文 夏立新 |
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| 作者单位： | 天津市黄瓜研究所,天津,300192;南开大学,生物化学与分子生物学系,天津,300071 |
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| 基金项目： | 天津市重点攻关项目(993122511-2) |
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| 摘 要： | 用RAPD技术对黄瓜基因组DNA进行多态性研究，分析了39份黄瓜材料的遗传差异。结果表明，所采用的172条随机引物中，有49条引物扩增谱带清晰且重复性较好，扩增总片段数达378个，单个引物的扩增片段数在4～12之间，片段大小在0．2～3．5kb之间。不同材料间的遗传距离(D)在0．064～0．592之间，并根据遗传距离，按UWPGA法进行了聚类分析。
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| 关 键 词： | 黄瓜 RAPD 遗传多样性 聚类分析 |
| 文章编号： | 1000-7091(2003)03-0050-03 |
| 修稿时间： | 2003-02-15 |
RAPD Analysis and Taxology Discussion on Cucumber Materials from Different Fountainhead Areas |
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| LIU Dian-lin,YANG Rui-huan,HA Yu-jie,CHEN De-fu,CHEN Xi-wen,XIA Li-xin. RAPD Analysis and Taxology Discussion on Cucumber Materials from Different Fountainhead Areas[J]. Acta Agriculturae Boreali-Sinica, 2003, 18(3): 50-52 |
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| Authors: | LIU Dian-lin YANG Rui-huan HA Yu-jie CHEN De-fu CHEN Xi-wen XIA Li-xin |
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| Abstract: | Random amplified Polymorphic DNA(RAPD) was used to analyze the genetic diversity among cucumber inbred line. The genetic of 39 cucumber inbred line was studied. The result showed that 49 of 172 arbitrary primers could amplify clear and repeatable bands. 398 fragments were obtained. Each primer gave 4-12 bands. The length of the band was 0.2-3.5 kb. The genetic distance(D) value between different breeds of cucumber inbred line was 0.064-0.592. The D value was used to construct a dendrogram by UPGMA. |
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| Keywords: | Cucumber RAPD Genetic diversity Cluster analysis |
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