不同光照强度对槲蕨叶绿素荧光参数·色素含量及抗性生理的影响

在不同光照条件下,测定了槲蕨孢子叶叶绿素荧光参数、叶绿素a、叶绿素b、叶绿素a+b、类胡萝卜素含量及过氧化物酶(POD)、过氧化氢酶(CAT)、丙二醛(MDA)和可溶性糖(SS)含量的变化。研究表明:在强光3 000 lx下Fo比1 0002、000 lx大,Fm各处理间相差不大,Fv/Fm在3 000 lx下明显减小。在1 000和2 000 lx下处理46、d后,ETR随光照强度的增加而增加,光抑制被消除。不同光照处理7d后3 000 lx叶绿素及类胡萝卜素含量下降幅度较大,1 000和2 000 lx差异不明显,POD和CAT活性、MDA、SS含量都在3 000 lx下最高。由此可见,3 000 lx光强对槲蕨生长不利,在实际生产中进行槲蕨人工栽培时应避免强光。

Effects of Different Light Intensities on the Chlorophyll Fluorescence Parameter, Pigment Contents and Resistance Physiology of Drynaria fortunei

Under different light intensities,the changes of chlorophyll fluorescence parameter,chlorophyll a,chlorophyll b,chlorophyll a b,carotenoid,malondialdhyde(MDA) and soluble sugar(SS) contents and peroxidase(POD) and catalase(CAT) activities were determined.The research showed that Fo under intense light of 3 000 lx was bigger than that under 1 000 and 2 000 lx,Fm had little difference among various treatments,and Fv/Fm reduced obviously under 3 000 lx.After treatment under 1 000 and 2 000 lx for 4 and 6 d,ETR increased along with the increment of light intensity,and the light inhibition was eliminated.After treatment with different illuminations for 7 d,the chlorophyll and carotenoid contents under 3 000 lx reduced more,the difference between 1 000 and 2 000 lx was not obvious,and both POD and CAT activities and MDA and SS contents were highest under 3 000 lx.This showed that the light intensity of 3 000 lx went against the growth of Drynaria fortunei,so the intense light should be avoided while conducting artificial cultivation of Drynaria fortunei in the practical production.