蓼科、禾本科植物细胞膜对铝胁迫反应的比较研究

以4种蓼科植物(荞麦、金荞麦、虎杖、无辣蓼)和2种禾本科植物(水稻、小麦)为材料,比较研究不同科属植物的细胞膜对铝胁迫反应的差异。结果表明:(1)6种植物叶片的游离脯氨酸含量、MDA含量和根系质膜透性均随着Al3 胁迫浓度增加而增大,禾本科植物的游离脯氨酸含量高于4种蓼科植物,荞麦、金荞麦的质膜透性、MDA含量高于虎杖、无辣蓼、水稻、小麦。(2)荞麦、小麦叶片的SOD和POD活性随Al3 胁迫浓度持续增长,虎杖和水稻持续下降,金荞麦和无辣蓼在25 mg/L Al3 处理时最高,100 mg/L Al3 胁迫明显降低。综合细胞膜对铝胁迫反应的各种特征,可以说明蓼科植物细胞膜透性变化是Al3 引发氧化胁迫的一种受损表现,而禾本科植物细胞膜透性变化是对A3 胁迫的一种适应性反应。

Comparism of Plasma Membrane Response to Al3+ Stress Between Polygonaceae Plants and Gramineae Plants

A comparism of plasma membrane response to Al~(3 ) stress between polygonaceae plants(Fagopyrum esculentum Moench,Fagopyrum dibotrys(D.Don) Hara,Polygonum cuspidatum Sieb.Et Zucc.,Polygonum pubescens Bl.) and gramineae plants(Oryza sativa L.,Triticum aestivum L.) was studied under controlled conditions with quartz sand culture.The results summarized as follows: Proline content,MDA content and membrane permeability in six plant species enhanced with increased Al~(3 ) concentration.Al~(3 ) triggered the evolution of proline in gramineae plants much higher than in polygonaceae plants,MDA content and membrane permeability were at a higher rate in Fagopyrum esculentum Moench and Fagopyrum dibotrys(D.Don) Hara than in the other four plant species.During the same period,the SOD and POD activity in leaf both increased in Fagopyrum esculentum Moench and Triticum aestivum L.,while decreased both in Polygonum cuspidatum Sieb.Et Zucc.and Oryza sativa L.SOD and POD activity in Fagopyrum dibotrys (D.Don) Hara and Polygonum pubescens Bl.raised with an increased of Al~(3 ) firstly(25 mg/L Al~(3 )),and then declined despite Al~(3 ) stressed concentration(100 mg/L Al~(3 )) still intensified.It was concluded that the change of membrane permeability in polygonaceae plants was a kind of cell damaged mechanism caused by oxidative stress with Al~(3 ),while in gramineae plants was a kind of defensive mechanism.