Changes of arbuscular mycorrhizal traits and community structure with respect to soil salinity in a coastal reclamation land |
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| Institution: | 1. State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, China;2. Environmental Resources and Agricultural Energy Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China;3. Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130012, China;1. Laboratorio de Biodiversidad Vegetal y Microbiana, Facultad de Cs Agrarias, Universidad Nacional de Rosario, Campo Exp. Villarino, Zavalla (2123), Argentina;2. Departamento Química Analítica, Facultad de Cs. Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario (2000), Argentina;3. Laboratorio de Micorrizas, Departamento de Cs. Químicas y Recursos Naturales, Universidad de La Frontera, Avda. Francisco Salazar 1145, Temuco (54-D), Chile;1. The Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin, China;2. Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, China;3. Institute of Natural Resources, Heilongjiang Academy of Sciences, Harbin, China |
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| Abstract: | A comprehensive knowledge on the relationship between soil salinity and arbuscular mycorrhizal fungi (AMF) is vital for a deeper understanding of ecosystem functioning under salt stress conditions. The objective of this study was to determine the effects of soil salinity on AMF root colonization, spore count, glomalin related soil protein (GRSP) and community structure in Saemangeum reclaimed land, South Korea. Soil samples were collected and grouped into five distinct salt classes based on the electrical conductivity of soil saturation extracts (ECse). Mycorrhizal root colonization, spore count and GRSP were measured under different salinity levels. AMF community structure was studied through three complementary methods; spore morphology, terminal restriction fragment length polymorphism (T-RFLP) and denaturing gradient gel electrophoresis (DGGE). Results revealed that root colonization (P < 0.01), spore count (P < 0.01) and GRSP (P < 0.01) were affected negatively by soil salinity. Spore morphology and T-RFLP data showed predominance of AMF genus Glomus in Saemangeum reclaimed land. T-RFLP and DGGE analysis revealed significant changes in diversity indices between non (ECse < 2 dS/m) and extremely (ECse > 16 dS/m) saline soil and confirmed dominance of Glomus caledonium only in soils with ECse < 8 dS/m. However, ribotypes of Glomus mosseae and Glomus proliferum were ubiquitous in all salt classes. Combining spore morphology, T-RFLP and DGGE analysis, we could show a pronounced effect in AMF community across salt classes. The result of this study improve our understanding on AMF activity and dominant species present in different salt classes and will substantially expand our knowledge on AMF diversity in reclaimed lands. |
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| Keywords: | Arbuscular mycorrhizal fungi Community structure DGGE Mycorrhizal traits Saemangeum reclaimed land Salinity T-RFLP |
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