| 基于 EST-SSR 标记的龟峰山杜鹃花种质资源的遗传多样性研究 |
| |
| 引用本文： | 李志良,程呈,张光望,方元平,金卫斌,王书珍. 基于 EST-SSR 标记的龟峰山杜鹃花种质资源的遗传多样性研究[J]. 农业科学与技术, 2016, 0(5): 1073-1076. DOI: 10.3969/j.issn.1009-4229.2016.05.010 |
| |
| 作者姓名： | 李志良 程呈 张光望 方元平 金卫斌 王书珍 |
| |
| 作者单位： | 大别山特色资源开发湖北省协同创新中心/黄冈师范学院生命科学学院,湖北黄冈,438000 |
| |
| 基金项目： | 湖北省教育厅科学技术研究项目(Q20152903);大学生创新创业训练项目(zx201425);《基因工程》教学模式的改革探索(xfg2015C22)。Supported by Scientific and Technological Research Project of Hubei Provincial Department of Education(Q20152903),National Col ege Students' Innovative Entrepreneurial Training Program(zx201425),Reform and Exploration of Teaching Mode of"Genetic Engineering"(xfg2015C22) |
| |
| 摘 要： | 杜鹃是杜鹃花科（ Ericaeae）杜鹃花属（Rhododendron）植物,具有较高的观赏价值和生态维持价值。该研究采用 EST-SSR标记对湖北龟峰山32个天然杜鹃花样品进行遗传多样性分析,旨在为龟峰山杜鹃的育种研究及新品种选育提供理论基础。7对EST-SSR引物共扩增出31条多态性条带,平均每个位点的等位基因数为4.43个,平均观察杂合度Ho和期望杂合度He的值分别为0.67958和0.72314。该研究开发的 EST-SSR标记不仅丰富了现有的 SSR数据库,也为后续分子标记辅助育种、遗传多样性和遗传结构分析、亲缘关系分析奠定了基础。
|
| 关 键 词： | 杜鹃花 微卫星 表达序列标签 遗传多样性 种质资源 |
EST-SSR Marker-based Genetic Diversity Analysis of Rhododendron simsii Germplasm in Guifeng Mountain |
| |
| Abstract: | Rhododendron simsi (Ericaeae: Rhododendron) has high ornamental value and ecological value. In this study, 7 pairs of novel EST-SSR markers were devel-oped from the genomic sequence of R. simsi , and they were used to investigate the genetic diversity of 32 natural R. simsi samples from Guifeng Mountain, Hubei Province. Results showed that a total of 31 polymorphic bands were amplified with al ele number per locus of 4.43. Mean values of heterozygosity (Ho) and expected heterozygosity (He) were 0.679 58 and 0.723 14, respectively. This research wil not only enrich the existing SSR database, but also lay a foundation for subsequent studies about molecular marker-assisted breeding, genetic diversity analysis, genetic structure analysis and phylogenetic analysis. |
| |
| Keywords: | Rhododendron simsi Microsatel ite Expressed sequence tag Genetic diversity Germplasm resource |
| 本文献已被 万方数据 等数据库收录！ |
|
