铜、锰诱导吉富罗非鱼血细胞凋亡及铁、锌的干预作用(英文)

为了研究重金属铜与锰对吉富罗非鱼血细胞凋亡的影响以及铁、锌的拮抗作用,采用原子吸收分光光度法检测血液及饲料中重金属的含量,采用流式细胞术检测实验鱼血细胞凋亡情况。360尾吉富罗非鱼幼鱼随机分为12组,每组30尾,分别以硫酸铜(0、2 000mg/kg)、硫酸锰(0、120 mg/kg)为攻毒组重金属源,以硫酸锌(20、320 mg/kg)、硫酸铁(150、350mg/kg)为拮抗组金属源,通过饲料投喂的方式进行血细胞凋亡的研究,养殖周期为20周。细胞凋亡分析结果表明,染铜组、染锰组实验鱼血细胞凋亡率显著高于对照组;补充铁和锌后,随着饲料添加铁、锌水平的增加,实验鱼血细胞凋亡率明显下降,但铁、锌干预组血细胞凋亡率显著高于对照组。试验表明,过量铜、锰可诱发实验罗非鱼血细胞凋亡;较高水平的铁、锌对铜、锰的毒性作用具有拮抗作用。

Effects of Copper and Manganese on Hemocyte Apoptosis and Antagonism of Iron and Zinc in Oreochromis niloticus

This study aimed to investigate the effects of copper and manganese on hemocyte apoptosis and the antagonism of iron and zinc in Oreochromis niloticus. The heavy metal contents in fish blood and feed were determined by atomic ab-sorption spectrophotometry, and the hemocyte apoptosis was determined by flow cytometry. A total of 360 tilapias were selected, and they were divided randomly and evenly into 12 groups. In the chal enge groups, the tilapias were fed with con-stant-level copper sulfate (0, 200 mg/kg) and manganese sulfate (0, 120 mg/kg); in the antagonism groups, the tilapias were fed with constant-level zinc sulfate (20, 320 mg/kg) and iron sulfate (150, 350 mg/kg). After 20-week aquaculture, the hemocyte apoptosis rates in the copper and manganese groups were significantly increased; with the increased addition levels of iron and zinc, the hemocyte apopto-sis rates in the iron and zinc groups were significantly reduced, but they were stil higher than that in the control group. In conclusion, excessive copper and man-ganese can cause apoptosis in hemocytes of O. niloticus. However, the toxic effects of copper and manganese can be antagonized by iron and zinc.