| 不同加倍方法处理玉米单倍体的加倍效果研究 |
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| 引用本文： | 刘俊,尹晓红,刘中杰,李建生. 不同加倍方法处理玉米单倍体的加倍效果研究[J]. 农业科学与技术, 2016, 0(5). DOI: 10.3969/j.issn.1009-4229.2016.05.016 |
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| 作者姓名： | 刘俊 尹晓红 刘中杰 李建生 |
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| 作者单位： | 1. 中国农业大学农学与生物技术学院,北京 100093; 丹东农业科学院,辽宁凤城 118109;2. 丹东农业科学院,辽宁凤城,118109;3. 中国农业大学农学与生物技术学院,北京,100093 |
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| 基金项目： | 国家转基因专项"抗玉米螟、抗粗缩病转基因玉米新品种选育"(2013ZX08003001-001-007)。Supported by National Major Program of Genetical y Modified Organisms for New Species Cultivation of China"Breeding of New Varieties of Genetical y Modified Maize Resistant to Borer and Rough Dwarf Disease"(2013ZX08003001-001-007) |
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| 摘 要： | 以不同基因型玉米材料诱导产生的单倍体幼苗为材料,在2~3叶期用0.06%浓度的秋水仙素配以2.0%的二甲基亚砜（DMSO）通过浸根、滴注心叶和针刺生长点3种不同的方法对单倍体进行人工化学加倍,并对不同加倍方法的加倍率和致死率进行方差分析和多重比较。结果表明,针刺生长点的方法加倍效果最好。平均加倍率为23%,结实率为21.4%,致死率为16.3%。与其他2种化学加倍方法比较,不仅加倍率显著提高,而且所用的秋水仙素剂量更低。
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| 关 键 词： | 玉米育种 单倍体 秋水仙素 染色体加倍 |
Doubling Efficiency of Maize Haploids Treated with Different Methods |
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| Abstract: | Haploid seedlings were inducted from different maize materials. At 2-3-leaf stage, maize haploids were treated with 0.06% colchicine and 2.0% dimethyl sulfox-ide (DMSO) by dipping root, dripping heart leaf and acupuncturing growing point, respectively. The doubling rate and mortality rate in different treatments were ana-lyzed by variance analysis and multiple comparisons. The result showed that grow-ing point acupuncturing method exhibited the highest doubling efficiency with an av-erage doubling rate of 23%, seed-setting rate of 21.4%, and mortality rate of 16.3%. Composed with other two chemical doubling methods, growing point acupuncturing method significantly improved the doubling rate of maize haploids with a lower ap-plication dose of colchicine. This study laid the foundation for industrial application of haploid breeding techniques. |
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| Keywords: | Maize breeding Haploid Colchicine Chromosome doubling |
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