Fluorodensitometric estimation of cucurbitacin-C in leaves ofCucumis sativus L. |
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Authors: | Herman A. Van Keulen |
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Affiliation: | (1) Institute for Horticultural Plant Breeding, Mansholtlaan 15, PO Box 16, 6700 AA Wageningen, The Netherlands |
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Abstract: | A quantitative method for the determination of cucurbitacin-C inCucumis sativus L. is presented. Crude chloroform extracts of the leaves were subjected to thin layer chromatography (TLC). After treatment with antimony trichloride and heating, the fluorescence of the spots was measured with a flying spot densitometer. |
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Keywords: | cucurbitacin-C Cucumis sativus L. TLC fluorodensitometry |
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