Construction and Identification of HSP70 Antisense RNA Expression Vector for Genetic Engineering Male Sterility in Plant

Objective]In order to study the relation between the HSP70 gene and male sterility of plant further.Methods]Anther specific expression promoter Osg6B of rice was coloned by PCR then connected with HSP70 antisense fragment to construct HSP70 antisense expression vector.The expression vector was identified by PCR experiment and enzyme digestion.Result]The sequence of coloned Osg6B promoter had 97% homology to the published sequence,and the cis-regulatory element in promoter area was integrated.HSP70 antisense expression vector driven by the promoter Osg6B was confired by colony PCR and enzyme digestion.Conclusion]The construction of expression vector would lay solid foundation for utilization of genetic engineering male sterility of plant.