辽宁省野生羊肚菌资源调查与遗传多样性分析

为了调查辽宁省的羊肚菌资源,了解其产地环境及种属的关系,探寻羊肚菌在辽宁省发生的适合条件,对辽宁省7市16个羊肚菌发生地开展了资源调查,共采集到羊肚菌样本53份。采用ITS和多基因系谱一致性系统发育种识别法(GCPSR),对采集到的羊肚菌样本进行鉴定和遗传多样性分析。结果表明,采集的羊肚菌样本被鉴定为5种,即Morchella sp.(Mes-6)、Morchella sp.(Mes-8)、Morchella sp.(Mes-9)、Morchella sp.(Mes-20)、Morchella sp.(Mes-21),其中,Mes-20和Mes-21为辽宁省新记录种。辽宁省的野生羊肚菌资源主要集中在朝阳市,以Mes-6为主。采用11条ISSR引物,共检测出多态位点51个。在居群水平上,Nei’s基因多样性为0.1201±0.0466,居群间遗传变异占总变异的60.79%,居群内占39.21%,遗传变异主要存在于居群间。

Resource Investigation and Genetic Diversity of Wild Morchella spp.in Liaoning Province

In order to investigate Morchella spp.resources to understand the environment of origin and species relations,find suitable conditions for Morchella spp.in Liaoning province.A habit survey of Morchella spp.was conducted in 7 cities,Liaoning,China.53 specimens were collected in 16 locations.By ITS and genealogical concordance phylogenetic species recognition(GCPSR),the samples were identified as Morchella sp.(Mes-6),Morchella sp.(Mes-8),Morchella sp.(Mes-9),Morchella sp.(Mes-20),Morchella sp.(Mes-21).Morchella sp.(Mes-20)and Morchella sp.(Mes-21)were first reported in Liaoning province.Wild Morchella spp.resources were mainly Mes-6 in Liaoning.Eleven ISSR primers were screened for the amplification of Morchella spp.,51 polymorpha bands were resulted.At the population level,Nei’s gene diversity was 0.1201±0.0466.The interpopulational variation accounted for 60.79%,and intrapopulational variation 39.21%.Moreover,the gene differentiation was existed in Morchella spp.interpopulations.