| 高效液相色谱法测定猪血浆中氟苯尼考的含量 |
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| 引用本文： | 魏海涛,方秋华,曾振灵,黄显会. 高效液相色谱法测定猪血浆中氟苯尼考的含量[J]. 广东畜牧兽医科技, 2010, 35(3): 31-33 |
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| 作者姓名： | 魏海涛 方秋华 曾振灵 黄显会 |
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| 作者单位： | 1. 华南农业大学兽医学院,广东,广州,510642;广东省动物保健品协会,广东,广州,510230
2. 华南农业大学兽医学院,广东,广州,510642 |
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| 摘 要： | 建立了猪血浆中氟苯尼考含量测定的高效液相色谱法(HPLC)。采用C18色谱柱(4.6mm×250mm,5μm),以乙腈-水(24:76)为流动相,流速1.0mL/min,检测波长223nm,进样量20μL,柱温为25℃。氟苯尼考浓度在0.1～16μg/mL时,标准曲线线性关系良好(r0.9998),无杂质干扰,回收率高于94%,批内变异系数和批间变异系数均低于5%。本方法操作简便,分析快速,结果准确,适用于猪血浆中氟苯尼考含量的测定及相关药代动力学研究。
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| 关 键 词： | 高效液相色谱法 氟苯尼考 猪 血浆 |
Content determination of florfenicol in pig plasma by HPLC |
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| Wei Haitao,Fang Qiuhua,Zeng Zhenling,Huang Xianhui. Content determination of florfenicol in pig plasma by HPLC[J]. Guangdong Journal of Animal and Veterinary Science, 2010, 35(3): 31-33 |
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| Authors: | Wei Haitao Fang Qiuhua Zeng Zhenling Huang Xianhui |
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| Affiliation: | 1 .College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642,China; 2.The Animal Health Products Association of Guangdong Province, Guangzhou 510230,China) |
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| Abstract: | A method for the content determination of florfenicol in pig plasma by HPLC was established. C18 column （4.6mm×250mm,5μ m） was used, acetonitrile-water （24：76） was used as mobile phase, the flow rate was 1.0mL/min, detection wavelength was 223nrn, the injection volume was 20μL and column temperature was 25 ℃ in HPLC system. There was no interference occurred from impurities. The calibration curve was linear at the range of 0.1 -16μg/mL （r 〉0.9998）, the recovery rate was above 94%. The intra-assay and inter-assay coefficient of variation were all below 5%. The method was rapid, simple and accurate for the content determination of florfenicol in pig plasma and the research on pharmacokinetics of florfenicol. |
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| Keywords: | HPLC florfenicol pigplasma |
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