乳脂合成前体对奶牛乳腺上皮细胞亚细胞蛋白质组学影响

向奶牛乳腺上皮细胞中添加β-羟基丁酸钠,利用双向电泳技术进行细胞亚细胞蛋白质组学方面的研究。结果表明,β-羟基丁酸钠对奶牛乳腺上皮细胞亚细胞蛋白表达有显著影响。Imagemaster6.0软件分析出4个表达上调的蛋白点,鉴定分别是ENO1、α-glucosidase、plastin3和ARPC2。通过实时荧光PCR检测以上蛋白在mRNA水平的表达差异,结果均有不同程度的提高,其中以ENO1、α-glucosidase及Arpc2提高显著(P<0.05)。研究所筛选出的蛋白,将有助于进一步揭示奶牛乳腺上皮细胞增殖和泌乳机制。

Effect of milk fat synthesis precursors on subcellular proteomics in bovine mammary epithelial

The purpose of this paper was to explore the impact of milk fat synthesis precursors on subcellular proteomics in bovine mammary epithelial cells by adding β-hydroxybutyrate to it,and 2-D electrophoresis technique was used for subcellular proteomics research.Experimental results showed that β-hydroxybutyrate sodium had a significant effect on the subcellular proteins in bovine mammary epithelial cells.By the software Imagemaster 6.0,we found that expression of four proteins rised,ENO1,α-glucosidase,plastin3 and ARPC2.Realtime PCR was used to test differentially expressed proteins,expressions rised at different extents,and ENO1,α-glucosidase,ARPC2 rised significantly(P<0.05).The proteins selected in this experiment would be helpful to further studies on bovine mammary epithelial cells proliferation and lactation.