四氯化碳致小鼠急性肝损伤动物模型建立方法的研究

通过对染毒途径、剂量和时间的研究,建立四氯化碳(CCl4)致小鼠急性肝损伤模型。采取腹腔注射和灌胃两种途径给予小鼠1%CCl4,染毒后24 h检测血清转氨酶含量,并观察肝脏病变。结果显示,灌胃组比腹腔注射组小鼠血清转氨酶变化个体差异小,肝脏病变明显,病灶分布均匀,且与实际中毒途径一致,故采用灌胃方法进行确定染毒剂量及时间试验。分别以0.125%、0.25%、0.35%、0.5%的CCl4给小鼠灌胃,染毒24 h后检测血清转氨酶含量,确定最佳染毒剂量。以该浓度给小鼠灌胃,分别于染毒后2、6、12、16、20、24、28、32、48 h检测小鼠血清转氨酶含量。结果表明,灌胃0.35%CCl4,小鼠血清转氨酶升高与对照组相比差异极显著(P<0.01),此浓度灌胃后,20 h血清转氨酶含量显著升高(P<0.01),24 h达到最高值,与对照组相比差异极显著(P<0.01)。因此,以0.35%四氯化碳,按0.1 mL.10 g-1体重灌胃,染毒24 h,可建立较理想的小鼠急性肝损伤模型。

Establishment of carbon tetrachloride-induced acute liver injury model in mice

Established the model of carbon tetrachloride-induced acute liver injury in mice by investigating the optimal exposure pathway,dose and time.Mice were prepared with 1% CCl4 by intraperitoneal injection and gavage respectively.Serum transaminase levels were detected and liver lesions were examined after 24 h.The results showed that individual difference of serum transaminase level in gavage group was smaller than intraperitoneal injection group,liver lesions were more uniform.Optimal dose and time was investigated by gavage because it has the same poisoning way with reality.Mice were given carbon tetrachloride in oil solution by gavage,with concentration of 0.125%,0.25%,0.35% and 0.5%.Detected serum transaminase levels after 24 h to measure optimal exposure dose.Serum transaminase levels was detected at 2,6,12,16,20,24,28,32,48 h after mice were expoed to optimal dose of CCl4.The results showed that mice received 0.35% CCl4 serum transaminase had significant difference compared with control group(P<0.01).Serum transaminase level had significant difference after 20 h(P< 0.05).The maximum turned out at 24 h,this level had significant difference compared with control group(P<0.01).The ideal model of acute liver injury in mice could be set up by gavage 0.35 % carbon tetrachloride,0.1 mL·10 g-1,for 24 h.