| 玉米大斑病菌特异性毒素组分的分离与纯化 |
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| 引用本文： | 张利辉,刘云惠,董金皋,李正平.玉米大斑病菌特异性毒素组分的分离与纯化[J].植物病理学报,2003,33(1):67-71. |
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| 作者姓名： | 张利辉 刘云惠 董金皋 李正平 |
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| 作者单位： | 河北农业大学真菌毒素实验室, 保定 071001 |
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| 基金项目： | 国家教育部骨干教师计划项目，河北省自然基金资助项目 (30 2 31 8) |
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| 摘 要： | 玉米大斑病菌的2号小种菌株用改良Fries培养液进行体外培养,培养滤液经-40℃冷冻干燥,加等体积甲醇去除沉淀后用乙酸乙酯提取,粗提物经HPLC C18柱可以分离出8个组分(峰3~10)。经生物测定发现,7号峰和10号峰的纯品对玉米叶片有明显的毒性,其中7号峰对带有Ht1基因的玉米表现出了一定的特异性。制备所得毒性组分分别进行红外光谱扫描,发现2个毒性组分的红外吸收光谱基本一致,吸收峰形状基本相同,只是其波数稍有变动。
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| 关 键 词： | 玉米大斑病 真菌毒素 高压液相色谱 红外光谱 |
| 文章编号： | 0412-0914(2003)01-0067-05 |
| 修稿时间： | 2001年12月13 |
Isolation and purification of specific toxin fractions produced by Exserohilum turcicum |
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| ZHANG Li-hui,LIU Yun-hui,DONG Jin-gao,LI Zheng-ping.Isolation and purification of specific toxin fractions produced by Exserohilum turcicum[J].Acta Phytopathologica Sinica,2003,33(1):67-71. |
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| Authors: | ZHANG Li-hui LIU Yun-hui DONG Jin-gao LI Zheng-ping |
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| Institution: | Mycotoxin Laboratory of Agricultural University of Hebei, Baoding 071001, China |
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| Abstract: | Crude toxins were extracted from culture filtrate of Exserohilum turcicum race 2 in modified Fries medium after freeze-dried at -40℃ and precipitated with methanol. Eight fractions (peak 3-10) were isolated from the crude toxins by means of HPLC analysis with C18 column. Peak 7 and 10 had distinct toxicity to the corn leaves and peak 7 had special toxicity to the corn with Ht1 resistant gene. Both the toxic fractions were scanned by infrared spectrum. It was shown that the two toxic fractions (peak 7 and 10) had analogous spectrum,similar shape of peaks,but a little difference in the wavenumbers. |
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| Keywords: | corn northern leaf blight mycotoxin HPLC infrared spectrum |
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