Simple species identification of Trichinella isolates by amplification and sequencing of the 5S ribosomal DNA intergenic spacer region |
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| Authors: | De Bruyne Aymeric Yera Hélène Le Guerhier Franck Boireau Pascal Dupouy-Camet Jean |
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| Affiliation: | Trichinella National Reference Center, Assistance Publique-H?pitaux de Paris, H?pital Cochin, R. Descartes University, 27 rue du Fbrg St. Jacques, 75014 Paris, France. |
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| Abstract: | We developed a PCR-based assay using a single primer pair to amplify the 5S ribosomal DNA intergenic spacer region to identify Trichinella isolates. In our method, amplified products are directly sequenced on both strands and compared to GenBank sequences. Using this method, we were able to identify Trichinella spiralis, T. britovi and T. nativa. This method permits rapid species identification of Trichinella isolates; however, further evaluation is required before recommending this approach for routine use. |
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