| 农杆菌素MI15的分离、纯化及分子量测定 |
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| 引用本文： | 赵艳景,张鹤龄. 农杆菌素MI15的分离、纯化及分子量测定[J]. 湖北农业科学, 2009, 48(10) |
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| 作者姓名： | 赵艳景 张鹤龄 |
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| 作者单位： | 江苏省海洋生物技术重点建设实验室,淮海工学院,江苏,连云港,222005;内蒙古大学生命科学学院,呼和浩特,010021 |
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| 基金项目： | 江苏省海洋生物技术重点建设实验室开放基金项目(HS07014):淮海工学院引进人才科研启动基金,淮海工学院自然科学基金 |
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| 摘 要： | 放射土壤杆菌(Agrobacterium radiobacter)MI15菌株.属于生物Ⅰ型.在平皿培养时,能够产生农杆菌素MI15,能抑制葡萄根癌土壤杆菌的生长.采用透析和DEAE纤维素柱离子交换层析纯化制备了MI15菌株分泌的农杆菌素MI15.经HPLC分析,此制备物纯度较高,可达到结构分析要求.紫外吸收光谱分析显示,该物质在260nm和280nm处均无洗脱峰,故认为该物质不是核酸、蛋白类似物.经红外吸收和元素分析,初步认定它是一种小分子有机物,质谱测定结果显示,农杆菌素MI15的分子量为368.
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| 关 键 词： | 农杆菌素MI15 层析 HPLC 质谱 |
Isolation,Purification,Molecular Weight Identification of Agrocin from Agrobaterium radiobacter MI15 |
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| ZHAO Yan-jing,ZHANG He-ling. Isolation,Purification,Molecular Weight Identification of Agrocin from Agrobaterium radiobacter MI15[J]. Hubei Agricultural Sciences, 2009, 48(10) |
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| Authors: | ZHAO Yan-jing ZHANG He-ling |
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| Abstract: | The MI15 strain of Agrobacterium radiobacter can produce Agrocin MI15 which inhibits the growth of A grobactium tumefacwns,a pathogen causing grape crown gall. Agrocin MI 15 was purified with dialysis followed by DEAE-cellulose column chromatography. HPLC analysis showed that a pure Agrocin MI 15 was obtained. UV spectrophotometric analysis indicated that the Agrocin MI 15 was not nucleic acid or protein-like substance because Agrocin MI 15 had no absorption spectrum at 260nm and 280nm. IR spectroscopy and element analysis proved Agrocin MI 15 to be a small molecular organic sub-strance. MS analysis indicated that the molecular weight of Agrocin MI 15 was 368. |
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| Keywords: | HPLC |
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