PLIN基因多态性与秦川牛胴体、肉质性状的相关性研究

旨在研究PLIN基因第3、4外显子多态性与秦川牛部分产肉性状的关系。以相同饲养条件下18~24月龄的405头秦川牛为研究对象,利用PCR-SSCP方法和测序技术,对PLIN基因第3、4外显子进行SNPs位点检测,并将发现的SNPs位点与部分产肉性状进行关联性分析。结果,在第3外显子的156 bp处发生T→C突变,第4外显子的14 bp处发生C→T突变。方差分析表明:AB基因型个体的宰前活体质量、胴体质量、背膘厚和眼肌面积显著高于AA和BB基因型个体(P0.05),而且相对于AA基因型个体达到差异极显著水平(P0.01);DE基因型个体的胴体长、胴体胸深和背膘厚显著高于DD和EE基因型个体(P0.05),DD基因型个体的肌肉嫩度极显著高于EE和DE基因型个体(P0.01)。研究显示,PLIN基因单核苷酸多态性对秦川牛脂肪沉积有一定的影响,可作为影响胴体和肉质的候选基因,指导肉牛的育种工作。

Study on the Relationship between Polymorphism of PLIN Gene and Carcass and Meat Quality Traits in Qinchuan Cattle

To reveal the polymorphism of exon3 and exon4 of PLIN gene in Qinchuan beef cattle and evaluate its relationship with production traits.405 Qinchuan cattle with 18-24 months age under identical feeding condition were selected.PCR-SSCP and sequencing method were applied in this study to detect the SNPs of PLIN exon3 and exon4,and analyze the correlation of SNPs with carcass and meat quality traits.T→C mutation at 156 base site in exon3 and C→T mutation at 14 base site in exon4 of this gene were detected.Correlation analysis showed that the index about slaughter weight,carcass weight,backfat thickness and loin muscle area of individual with AB genotype were significant higher than those with AA and BB genotypes(P<0.05) ,and compared to individual with AA genotype was very significantly different(P<0.01).This analysis also showed that the carcass length,carcass chest depth and backfat thickness of individual with DE genotype were significant higher than those with EE and DD genotypes(P<0.05) ,and compared to individual with EE and DE genotypes,the tenderness of individual with DD genotype was very significantly different(P<0.01).The results indicated that the SNPs of PLIN gene had effect on the capacity of fat accumulate of Qinchuan cattle,it could be a candidate gene for beef modify and directed to the beef cattle breeding.