The effect of IGF-1 and FSH on the in vitro development of caprine secondary follicles and on the IGF-1, IGFR-I and FSHR mRNA levels |
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| Authors: | Brito I R Saraiva M V A Araújo V R Celestino J J H Magalhães-Padilha D M Lima I M T Van den Hurk R Figueiredo J R Silva J R V |
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| Affiliation: | LAMOFOPA, Universidade Estadual do Ceará, Avenida Paranjana, 1700, Itaperi, CEP: 60740-930, Fortaleza, CE, Brazil. ivinabrito@yahoo.com.br |
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| Abstract: | This study verified the in vitro effects of IGF-1, FSH or both on caprine preantral follicle development and mRNA levels encoding IGF-1, IGFR-1 and FSHR. Secondary follicles were cultured for six days with FSH, IGF-1 or IGF-1+FSH. The results showed that IGF-1 and/or FSH addition did not influence follicular development for six days. The interaction between IGF-1 and FSH increased the mRNA levels of IGF-1 and FSHR, and FSH increased the expression of the IGFR-1 mRNA. Thus, IGF-1 and/or FSH increased IGF-1, IGFR-1 and FSHR mRNA levels in in vitro cultured caprine secondary follicles, but they did not influence their development after six days of in vitro culture. |
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