| 一株土著B[a]P降解菌的筛选及降解特性研究 |
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| 引用本文： | 顾平,周启星,王鑫,伍跃辉,陈威,张倩茹,龚起,周爱申. 一株土著B[a]P降解菌的筛选及降解特性研究[J]. 农业环境科学学报, 2018, 37(5): 926-932 |
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| 作者姓名： | 顾平 周启星 王鑫 伍跃辉 陈威 张倩茹 龚起 周爱申 |
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| 作者单位： | 黑龙江省环境监测中心站;中国科学院沈阳应用生态研究所;南开大学环境科学与工程学院环境污染过程与基准教育部重点实验室/天津市城市生态环境修复与污染防治重点实验室;黑龙江省环境应急与事故调查中心 |
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| 基金项目： | 中央高校基本科研业务费专项资金项目 |
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| 摘 要： | 通过在液体培养基中添加苯并[a]芘(B[a]P)作为唯一碳源反复驯化培养,从长期受石油烃和多环芳烃(PAHs)污染的土壤中分离出8株能够降解B[a]P的细菌,其中一株细菌(命名为BB-1)具有最大降解效果。采用16s RNA基因测序结果表明,BB-1与巨大芽孢杆菌(Bacillus megaterium)同源性为100%。为了研究BB-1的降解特性,在pH7.0、30℃条件下培养8 d,菌株B[a]P的降解率高达66.36%。为了考察培养基初始pH和外加蔗糖浓度对BB-1降解B[a]P的影响,30℃下振荡培养8 d,当设置培养基初始pH为4.0、6.0、8.0和10.0时,BB-1对B[a]P的降解率分别为12.14%、39.61%、55.21%和30.03%,可见pH为8.0时其降解效果最优;当添加0.1%蔗糖和0.5%蔗糖为外加碳源时,菌株BB-1对B[a]P的降解率分别为70.56%和74.89%,表明0.5%蔗糖的降解效果最优。
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| 关 键 词： | PAHs B[a]P 污染土壤 生物降解 |
| 收稿时间： | 2017-07-06 |
The screening-out and degradation character of an indigenous benzo[a]pyrene-degrading bacteria |
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| GU Ping,ZHOU Qi-xing,WANG Xin,WU Yue-hui,CHEN Wei,ZHANG Qian-ru,GONG Qi and ZHOU Ai-shen. The screening-out and degradation character of an indigenous benzo[a]pyrene-degrading bacteria[J]. Journal of Agro-Environment Science( J. Agro-Environ. Sci.), 2018, 37(5): 926-932 |
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| Authors: | GU Ping ZHOU Qi-xing WANG Xin WU Yue-hui CHEN Wei ZHANG Qian-ru GONG Qi ZHOU Ai-shen |
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| Affiliation: | Heilongjiang Provincial Environmental Monitoring Center Station, Harbin 150056, China;Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China,Key Laboratory of Pollution Processes and Environmental Criteria Ministry of Education/Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China,Key Laboratory of Pollution Processes and Environmental Criteria Ministry of Education/Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China,Heilongjiang Provincial Environmental Monitoring Center Station, Harbin 150056, China,Heilongjiang Provincial Environmental Monitoring Center Station, Harbin 150056, China,Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China,Environmental Emergency and Accident Investigation Center of Heilongjiang Province, Harbin 150090, China and Heilongjiang Provincial Environmental Monitoring Center Station, Harbin 150056, China |
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| Abstract: | |
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| Keywords: | polycyclic aromatic hydrocarbons (PAHs) benzo[a]pyrene (B[a]P) contaminated soil biodegradation |
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