METFORMIN短期内对蛋鸡体AMPK活性的影响

选用日龄、体重及产蛋率相近的产蛋高峰期的新罗曼蛋鸡20只，按两两配对的原则，分为2个处理组，每个处理5个重复，每个重复2只鸡；其中实验组蛋鸡日粮中含有0．8％浓度的Metformin；配对组蛋鸡采食日粮除不合Metformin外，日粮成分与实验组相同，并按实验组前1d的采食量供给；正式实验期为5d。实验组与配对组相比，实验组蚤鸡肝脏中AMPK活性提高了24．82％（P〈0．3），而卵巢中AMPK活性仅提高了10．13％（P〈0．6）；实验组蛋鸡肝脏中TG下降了13．61％（P〈0．1）；血清中VLDL—C含量下降了12．66％（P〈0．6），同时在直径分别为2，1，0．5cm的卵泡中，卵黄胆固醇含量分别下降了9．18％（P〈0．01），9．72％（P〈0．01），17．04％（P〈0．05）。实验结果表明Metformin提高了蛋鸡体内AMPK的活性，并降低了蛋鸡体内脂质的合成及卵黄中胆固醇的含量。

EFFECTS OF METFORMIN ON AMP-ACTIVATED PROTEIN KINASE AND LIPID METABOLISM IN LAYING HENS

Twenty New Roman laying hens of similar age,weight and laying performance were divided into two groups of five replicates,with two hens each.For 5 successive days,the experimental group were fed diet containing 0.8% metformin,while the control group were fed the same diet without metformin.Compared with the control group,the experimental group showed a 24.82%(P<0.3%) and(10.13%)(P<0.6%) increase,respectively,in AMPK activity in their liver and ovary.Their TG content in the liver and VLDL-C in the blood serum decreased by 13.61%(P<0.1) and 12.66%(P<0.6%),respectively.Cholesterol content in the ovary blastula with a diameter of 2 cm,1 cm and 5 cm decreased by 9.18%(P<0.01),9.72%(P<0.01) and 17.04%(P<0.05),respectively.These results suggest that feeding diet with metformin for 5 days could activate AMPK activity of laying hens and decrease the amount of lipid synthesis and cholesterol in the yolk.