大球盖菇多糖的分子质量分布及其单糖的组成

 【目的】从大球盖菇(Stropharia rugoso-annulata)中提取多糖，并对其分子质量及组成糖进行研究。【方法】采用水提法提取大球盖菇多糖，利用高效液相色谱法研究大球盖菇多糖分子质量分布及提取过程中多糖分子质量的变化，并采用红外光谱、核磁共振法对其组成糖进行研究。【结果】大球盖菇多糖水溶性多糖类物质的分子质量分布于22 kD附近；大球盖菇多糖同时存在α和β糖苷键，以吡喃糖为主，由5种单糖构成，其中有D-果糖、D-葡萄糖和D-木糖。【结论】以稀盐溶液为流动相的检测手段更能真实地反映大球盖菇多糖的分子质量分布情况，大球盖菇多糖是由5种单糖组成的杂多糖。

Distribution of Stropharia rugoso-annulata Polysaccharides Molecular Weight and Component Sugar

【Objective】The aim of this paper was to extract and isolate Stropharia rugoso-annulata polysaccharides and analyze the molecular weight and component sugar of polysaccharides.【Method】In this paper, polysaccharide from Stropharia rugoso-annulata was obtained by water extraction method, which the molecular weight distribution and changes during extraction course was investigated by using high performance liquid chromatography. The component sugar of the polysaccharides were also measured by infrared spectrum and nuclear magnetic resonance methods. 【Result】The molecular weight distribution of water soluble polysaccharides from Stropharia rugoso-annulata was about 22 kD, α and β glucosidic bonds were co-exist in polysaccharides molecule which contain 5 kinds of monosaccharides. The main monosaccharide was pyranose. D-fructose, D-glucose and D-xylose exist in polysaccharides molecule. 【Conclusion】 The detection method with hydrochloric acid solution as the mobile phase can reflect more real molecular weight distribution of polysaccharide from Stropharia rugoso-annulata, and Stropharia rugoso-annulata polysaccharide is a heterosaccharide which is composed of five monosaccharides.