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酶法提取稻谷加工副产物碎米中蛋白成分工艺的研究
引用本文:朱建华,全小丽,黃伟强.酶法提取稻谷加工副产物碎米中蛋白成分工艺的研究[J].江西农业学报,2012(8):98-99,102.
作者姓名:朱建华  全小丽  黃伟强
作者单位:韶关学院英东食品科学与工程学院;广东金友集团有限公司
基金项目:广东省韶关市科技计划项目[韶科(管)2010-02];广东省教育部产学研结合项目(2011B090600027)
摘    要:通过单因素实验和正交实验,用中性蛋白酶水解法对稻谷加工副产物碎米进行了蛋白质的提取研究,得到如下最佳提取条件:酶解时间2.5 h、酶解温度55℃、酶的添加量1.0%(w/w)、液固比10∶1。在此条件下蛋白质的提取率可达74.8%。

关 键 词:碎米  蛋白质  中性蛋白酶  提取率  工艺

Study on Technology of Extracting Protein from Cereal Processing By-product Broken Rice with Enzyme Method
ZHU Jian-hua,QUAN Xiao-li,HUANG Wei-qiang.Study on Technology of Extracting Protein from Cereal Processing By-product Broken Rice with Enzyme Method[J].Acta Agriculturae Jiangxi,2012(8):98-99,102.
Authors:ZHU Jian-hua  QUAN Xiao-li  HUANG Wei-qiang
Institution:1(1.Yingdong College of Food Science and Engineering,Shaoguan University,Shaoguan 512005,China; 2.Guangdong Jinyou Group Limited Company,Shaoguan 512005,China)
Abstract:The technology of extracting protein from cereal processing by-product broken rice with neutral protease hydrolysis method was investigated through single-factor experiment and orthogonal experiment.The optimum extraction conditions were acquired as follows: enzymolysis time 2.5 h,enzymolysis temperature 55 ℃,enzyme addition amount 1.0%(w/w) and the ratio of liquid to solid 10∶1.Under the above optimal conditions,the protein extraction rate reached 74.8%.
Keywords:Broken rice  Protein  Neutral protease  Extraction rate  Technology
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