| 猪场粪污中大肠埃希菌磺胺类耐药基因型及表型分析 |
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| 引用本文： | 魏勇,陈美琪,王利,肖云,于吉锋,李兴玉,LI Xingyu. 猪场粪污中大肠埃希菌磺胺类耐药基因型及表型分析[J]. 动物医学进展, 2016, 0(11). DOI: 10.3969/j.issn.1007-5038.2016.11.012 |
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| 作者姓名： | 魏勇 陈美琪 王利 肖云 于吉锋 李兴玉 LI Xingyu |
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| 作者单位： | 1. 四川省畜牧科学研究院，四川成都，610066;2. 西南民族大学生命科学与技术学院,四川成都,610041;3. 四川省大竹县畜牧局,四川大竹,635100;4. Sichuan Animal Science Academe,Chengdu,Sichuan,610064,China |
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| 基金项目： | 四川生猪产业技术体系创新团队项目(sccxtd-004)；四川省富民强县项目(邻水县优质生猪健康养殖关键技术集成与产业化示范，2014) |
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| 摘 要： | 为了检测猪场粪污中大肠埃希菌对磺胺类药物的耐药状况,用美国临床和实验室标准协会(CLSI)推荐的药敏纸片扩散法检测11株猪场粪污中大肠埃希菌对磺胺类药物的耐药性表型,用multi-PCR Kit检测磺胺类药物的耐药基因(sul1,sul2,sul3)。结果表明,11株猪场粪污中大肠埃希菌对磺胺复合物的耐药率为45%,磺胺二甲基恶唑耐药率为45%,磺胺甲基恶唑耐药率为64%。磺胺类药物的Sul 1基因检出率为100%,sul 2基因检出率为90.9%,sul 3基因检出率为36.4%,磺胺类抗生素耐药基因的总检出率为100%。研究结果可为大肠埃希菌的耐药性研究以及磺胺类耐药机制的研究提供科学依据。
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| 关 键 词： | 猪场粪污 大肠埃希菌 磺胺类 耐药基因 |
Detection of Drug Resistance Phenotypes and Genotypes of Sulfonamides in Escherichia coli Isolated from Piggery Wastes |
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| Abstract: | In order to understand the drug resistance of sulfonamides in Escherichia coli isolated from pig-gery wastewater,eleven isolates of E.coli were analyzed by K-B method according to the recommendation of the CLSI.Then,multi-PCR Kit method was used to detect sulfonamides-resistant genes(sul1,sul2, sul3).The result shows that resistant rates of eleven E.coli isolates to peptidylsulfanilamide,sulfamoxole and sulfamethoxazole were 45%,45% and 64% respectively.The detection rates of sul 1,sul 2 and sul 3 gene were 100%,90.9% and 36.4%,respectively.The detection rate of all sulfonamide resistance genes was 100%.It will benefit the further study of drug-resistant mechanism of E.coli. |
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| Keywords: | E.coli sulfonamide drug resistance gene piggery waste |
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