益生菌抵御大鼠急性腹泻的效果及其对肠道黏膜屏障的影响

本试验旨在研究酪酸梭菌或唾液乳酸菌对预防急性腹泻、保护肠道黏膜屏障的作用效果。选取清洁级21日龄断奶Wistar大鼠平均体重(57.45±6.23)g]48只,随机分在4个组:正常对照组(C1组)、模型对照组(C2组)、酪酸梭菌组(T1组)、唾液乳酸菌组(T2组)。每个组6个重复,每个重复2只大鼠。试验期共9 d,C1、C2组经口灌服生理盐水,T1、T2组分别灌服酪酸梭菌和唾液乳酸菌。第8天开始除C1组外,其余各组灌服番泻叶制剂致泻,记录各组大鼠腹泻情况。试验结束后处死所有大鼠并采集血液及肠道样品。结果显示:1)灌服番泻叶后各组大鼠均出现腹泻。C2组稀便率、腹泻指数、血清内毒素及D-乳酸含量极显著高于C1组(P0.01);而与C2组相比,T1、T2组腹泻指数、血清内毒素及D-乳酸含量均极显著降低(P0.01)。2)与C2组相比,T1、T2组隐窝深度显著降低(P0.05),绒毛高度与隐窝深度的比值极显著升高(P0.01),且T1组回肠闭锁小带蛋白1(ZO-1)、闭锁蛋白(occludin)mRNA的相对表达量分别极显著(P0.01)和显著(P0.05)升高。3)C2组回肠白细胞介素-10(IL-10)、白细胞介素-1β(IL-1β)mRNA的相对表达量分别极显著(P0.01)和显著(P0.05)高于C1组;而与C2组相比,T1组回肠IL-10 mRNA的相对表达量极显著降低(P0.01)。以上结果说明,在本试验中,酪酸梭菌与唾液乳酸菌在一定程度上可以缓解由番泻叶引起的大鼠急性腹泻,降低肠道通透性,同时具有增强和保护肠道黏膜屏障的作用。

Effects of Probiotics to Against Acute Diarrhea and on Intestinal Mucosal Barrier of Rats

The purpose of this study was to evaluate the effects of Clostridium butyricum or Lactobacillus sali?varius ZJ614 on prevention of acute diarrhea and protection of intestinal mucosal barrier function. Forty?eight 21?day?weanned Wistar rats with the average body weight of (57.45±6.23) g were selected and randomly di?vided into four groups:normal control group ( C1 group) , model control group ( C2 group) , Clostridium bu?tyricum group ( T1 group) and Lactobacillus salivarius ZJ614 group ( T2 group) . Each group had six repli?cates, and each replicate had two rats. This experiment lasted for 9 days. C1 and C2 groups were orally admin?istered saline, and T1 and T2 groups were administered probiotics accordingly. On the 8th day, every group except C1 group was administered folium senna to cause acute diarrhea and then record diarrhea condition of rats. All rats were sacrificed, and blood and intestinal samples were collected at the end of experiment. The re?sults showed as follows:1) all rats displayed diarrhea symptoms as long as administered folium senna. Loose stools rate, diarrhea index, serum endotoxin and D?lactic acid contents were significantly increased in C2 group compared with C1 group ( P<0.01) . The diarrhea index, serum endotoxin and D?lactic acid contents decreased significantly in T1 and T2 groups compared with C2 group ( P<0.01) . 2) Compared with C2 group, the crypt depth in T1 and T2 groups was significantly decreased (P<0.05), the ratio of villus height to crypt depth in T1 and T2 groups was significantly increased ( P<0.01) , and the relative expression levels of ileum zonula oc?cludens?1 ( ZO?1) ( P<0.01) and occludin mRNA ( P<0.05) in T1 group were significantly improved. 3) C2 group significantly up?regulated the relative expression levels of ileum interleukin?10 (IL?10) (P<0.01)and interleukin?1β( IL?1β) mRNA ( P<0.05) compared with C1 group, and T1 group down?regulated the relative expression level of IL?10 mRNA compared with C2 group ( P<0.01) . In summary, Clostridium butyricum or Lactobacillus salivarius ZJ614 can alleviate the actue diarrhea induced by senna on a certain degree, and also decrease the intestinal permeability, and enhance intestinal mucosal barrier.