蛋鸡感染新城疫病毒后输卵管组织中病毒分布与炎症相关细胞因子mRNA的表达

通过间接免疫荧光(IFC)和实时荧光定量PCR(real-time PCR)方法研究了蛋鸡感染新城疫病毒(NDV)后输卵管组织中病毒分布及炎症相关细胞因子mRNA的表达变化。结果表明,NDV可在输卵管组织中复制,证明感染成功,NDV感染引起蛋鸡输卵管膨大部和子宫部IL-2、IL-6、IL-1β、IFN-β、CXCLi1、CXCLi2及CCR5mRNA mRNA表达上调;在感染120h后,膨大部IL-2、IFN-β和IL-1βmRNA表达达到峰值,分别为健康对照组的137倍、95.1倍和15.9倍;子宫部在感染第9天达到峰值,分别为36.2倍、59.9倍和10.3倍。在感染前3d,IFN-αmRNA在膨大部的表达为下调的趋势,而从感染第5天开始上调;而子宫部IFN-α的mRNA表达均上调。NDV感染后趋化因子CXCLi1、CXCLi2及CCR5mRNA的在膨大部和子宫部表达均上调,其中膨大部CXCLi1、CXCLi2和CCR5mRNA上调的最高值分别为20.5倍、78.3倍和22.1倍;其在子宫部分别上调5.5倍、64.3倍、25.5倍。总的来说,NDV能感染蛋鸡输卵管组织并引起IL-2、IL-6、IL-1β、IFN-β、CXCLi1、CXCLi2及CCR5mRNA表达上调,这些细胞因子mRNA表达的上调可能与输卵管炎症的发生有关。

Virus Distributions and Related Cytokine mRNA Expressions in Oviducts of Layers Infected with Newcastle Disease Virus

To investigate the main cytokine mRNA expression changes in oviducts of layers infected with NDV,real-time PCR was used to detect the mRNA expression changes of IL-2,IL-6,IL-1β,IFN-β,CXCLi1,CXCLi2 and CCR5 in magnum and uterus.The results showed that the cytokine and chemokine mRNA expressions of IL-2,IL-6,IL-1β,IFN-β,CXCLi1,CXCLi2 and CCR5 mRNA were up-regulated in magnum and uterus after NDV infection. At the fifth day post infection(dpi),the mRNA expressions of IL-2,IFN-β,IL-1βreached peak,the changes were 137-fold,15.9-fold and 95.1-fold respectively compared with healthy controls;while the mRNA expression chan-ges of IL-2,IFN-β,IL-1βpeaked at 9 dpi in uterus,respectively 36.2-fold,59.9-fold and 10.3-fold.IFN-αmRNA expressions in magnum were down-regulated during the three days post infection,while at 5dpi,it began to up reg-ulate.In uterus,IFN-αmRNA expression was always up-regulated during infection process.CXCLi1,CXCLi2 and CCR5 mRNA were up-regulated in magnum and uterus after NDV infection,CXCLi1,CXCLi2,CCR5 mRNA were up-regulated in magnum,the peak values were 20.5-fold,78.3-fold and 22.1-fold,respectively.In general,oviuctal tissues can be infected with NDV,and IL-2,IL-6,IL-1β,IFN-β,CXCLi1,CXCLi2 and CCR5 mRNA expressions were induced by NDV infection.The induction of mRNA expressions of the cytokines and chemokines may be as-sociated with the inflammation occurred in oviduct tissues after NDV infection.