| 猪生长激素基因第二外显子区遗传变异的序列基础 |
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| 引用本文： | 姜志华 Rott.,OJ. 猪生长激素基因第二外显子区遗传变异的序列基础[J]. 南京农业大学学报, 1997, 20(2): 67-71 |
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| 作者姓名： | 姜志华 Rott. OJ |
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| 作者单位： | 南京农业大学动物科技学院，慕尼黑科技大学动物遗传育种研究所 |
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| 基金项目： | 国家教委优秀青年教师资助 |
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| 摘 要： | 采用聚合酶链反应（ＰＣＲ）及ＰＣＲ产物有直接序列分析方法，对猪一长激素基因第二外显子外遗传变异的序列基础进行了研究，ＰＣＲ扩增片段长度为２３０ｂｐ，包含全部的生长激素基因的第二外显子序列（１６１ｂｐ）共分析了６头二花脸和６头长白猪，结果表明：在猪的生长激素基因第二外显子子区，共有７处碱基替换，依次为５０７处的Ｃ／Ｔ替换，５２０处的Ｃ／Ｔ替换，５４６处的Ｇ／Ａ替换，５５５处的Ｇ／Ａ替换，５５６处的Ｃ
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| 关 键 词： | 猪 生长激素基因 第二外显子 序列 |
SEQUENCE CHARACTERIZATION OF GENETIC POLYMORPHISMS AT THE 2ND EXON OF PORCINE GROWTH HORMONE GENE |
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| Jiang Zhihua ,O.J.Rottmann and F. Pirchner. SEQUENCE CHARACTERIZATION OF GENETIC POLYMORPHISMS AT THE 2ND EXON OF PORCINE GROWTH HORMONE GENE[J]. Journal of Nanjing Agricultural University, 1997, 20(2): 67-71 |
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| Authors: | Jiang Zhihua O.J.Rottmann F. Pirchner |
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| Affiliation: | Jiang Zhihua 1,O.J.Rottmann 2 and F. Pirchner 2 |
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| Abstract: | The genetic polymorphisms at the 2nd exon of porcine growth hormone (pGH) gene were characterized in 6 Erhualian and 6 Landrace pigs with direct sequencing a 230 bp fragment which was amplified by PCR. This fragment included the entire 2nd exon of pGH gene, being 161 bp. The results showed that base substitutions occurred at 7 positions within the exon: C/T at 507, C/T at 520, G/A at 546, G/A at 555, C/A at 556, C/T at 595 and C/T at 634 respectively. The base substitutions at 507, 546 and 555 556 cause amino acid changes in the amino acid sequence of pGH, while others were silent. The restriction enzyme maps analysed with GCG program showed that the base substitutions which cause the amino acid changes, can be identified with digestions of different restriction enzymes. |
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| Keywords: | pig growth hormone gene the 2nd exon sequence mutation |
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